untitled
<OAI-PMH schemaLocation=http://www.openarchives.org/OAI/2.0/ http://www.openarchives.org/OAI/2.0/OAI-PMH.xsd>
<responseDate>2018-01-15T18:36:34Z</responseDate>
<request identifier=oai:HAL:pasteur-00782031v1 verb=GetRecord metadataPrefix=oai_dc>http://api.archives-ouvertes.fr/oai/hal/</request>
<GetRecord>
<record>
<header>
<identifier>oai:HAL:pasteur-00782031v1</identifier>
<datestamp>2017-12-21</datestamp>
<setSpec>type:ART</setSpec>
<setSpec>subject:sdv</setSpec>
<setSpec>collection:INSERM</setSpec>
<setSpec>collection:UNIV-AG</setSpec>
<setSpec>collection:MNHN</setSpec>
<setSpec>collection:RIIP_DAKAR</setSpec>
<setSpec>collection:RIIP</setSpec>
<setSpec>collection:RIIP_GUADELOUPE</setSpec>
<setSpec>collection:UNIV-REUNION</setSpec>
<setSpec>collection:UNIV-PARIS7</setSpec>
<setSpec>collection:USPC</setSpec>
</header>
<metadata><dc>
<publisher>HAL CCSD</publisher>
<title lang=en>Survey of Naegleria fowleri in Geothermal Recreational Waters of Guadeloupe (French West Indies).</title>
<creator>Moussa, Mirna</creator>
<creator>De Jonckheere, Johan F</creator>
<creator>Guerlotté, Jérôme</creator>
<creator>Richard, Vincent</creator>
<creator>Bastaraud, Alexandra</creator>
<creator>Romana, Marc</creator>
<creator>Talarmin, Antoine</creator>
<contributor>Institut Pasteur de la Guadeloupe ; Réseau International des Instituts Pasteur (RIIP) - Institut Pasteur de la Guadeloupe</contributor>
<contributor>De Duve Institute ; de Duve Institute</contributor>
<contributor>Scientific Institute of Public Health ; Scientific Institute of Public Health</contributor>
<contributor>Université des Antilles et de la Guyane (UAG)</contributor>
<contributor>Muséum National d'Histoire Naturelle (MNHN)</contributor>
<contributor>Institut Pasteur de Dakar ; Institut Pasteur de Dakar - Réseau International des Instituts Pasteur (RIIP)</contributor>
<contributor>Protéines de la membrane érythrocytaire et homologues non-érythroides ; Université des Antilles et de la Guyane (UAG) - Institut National de la Transfusion Sanguine [Paris] (INTS) - Université Paris Diderot - Paris 7 (UPD7) - Université de la Réunion (UR) - Institut National de la Santé et de la Recherche Médicale (INSERM)</contributor>
<contributor>ACIP (A-01-2011)</contributor>
<description>International audience</description>
<source>ISSN: 1932-6203</source>
<source>PLoS ONE</source>
<publisher>Public Library of Science</publisher>
<identifier>pasteur-00782031</identifier>
<identifier>https://hal-riip.archives-ouvertes.fr/pasteur-00782031</identifier>
<identifier>https://hal-riip.archives-ouvertes.fr/pasteur-00782031/document</identifier>
<identifier>https://hal-riip.archives-ouvertes.fr/pasteur-00782031/file/article_naegleria_plos_one.pdf</identifier>
<source>https://hal-riip.archives-ouvertes.fr/pasteur-00782031</source>
<source>PLoS ONE, Public Library of Science, 2013, 8 (1), pp.e54414. 〈10.1371/journal.pone.0054414〉</source>
<identifier>DOI : 10.1371/journal.pone.0054414</identifier>
<relation>info:eu-repo/semantics/altIdentifier/doi/10.1371/journal.pone.0054414</relation>
<identifier>PUBMED : 23349880</identifier>
<relation>info:eu-repo/semantics/altIdentifier/pmid/23349880</relation>
<language>en</language>
<subject>[SDV.MP.BAC] Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology</subject>
<type>info:eu-repo/semantics/article</type>
<type>Journal articles</type>
<description lang=en>In 2008 a fatal case of primary amoebic meningoencephalitis, due to the amoeboflagellate Naegleria fowleri, occurred in Guadeloupe, French West Indies, after a child swam in a bath fed with geothermal water. In order to improve the knowledge on free-living amoebae in this tropical part of France, we investigated on a monthly basis, the presence of Naegleria spp. in the recreational baths, and stream waters which feed them. A total of 73 water samples, 48 sediments and 54 swabs samples were collected from 6 sampling points between June 2011 and July 2012. The water samples were filtered and the filters transferred to non-nutrient agar plates seeded with a heat-killed suspension of Escherichia coli while sediment and swab samples were placed directly on these plates. The plates were incubated at 44°C for the selective isolation of thermophilic Naegleria. To identify the Naegleria isolates the internal transcribed spacers, including the 5.8S rDNA, were amplified by polymerase chain reaction and the sequence of the PCR products was determined. Thermophilic amoebae were present at nearly all collection sites. The pathogenic N. fowleri was the most frequently encountered thermophilic species followed by N. lovaniensis. The concentration of N. fowleri was rather low in most water samples, ranging from 0 to 22 per liter. Sequencing revealed that all N. fowleri isolates belonged to a common Euro-American genotype, the same as detected in the human case in Guadeloupe. These investigations need to be continued in order to counsel the health authorities about prevention measures, because these recreational thermal baths are used daily by local people and tourists.</description>
<date>2013</date>
</dc>
</metadata>
</record>
</GetRecord>
</OAI-PMH>
