untitled
<OAI-PMH schemaLocation=http://www.openarchives.org/OAI/2.0/ http://www.openarchives.org/OAI/2.0/OAI-PMH.xsd>
<responseDate>2018-01-15T18:26:53Z</responseDate>
<request identifier=oai:HAL:hal-01209365v1 verb=GetRecord metadataPrefix=oai_dc>http://api.archives-ouvertes.fr/oai/hal/</request>
<GetRecord>
<record>
<header>
<identifier>oai:HAL:hal-01209365v1</identifier>
<datestamp>2018-01-11</datestamp>
<setSpec>type:POSTER</setSpec>
<setSpec>subject:sdv</setSpec>
<setSpec>collection:CNRS</setSpec>
<setSpec>collection:UNIV-RENNES1</setSpec>
<setSpec>collection:INRA</setSpec>
<setSpec>collection:UNAM</setSpec>
<setSpec>collection:IRSET</setSpec>
<setSpec>collection:AGROCAMPUS-OUEST</setSpec>
<setSpec>collection:BIOSIT</setSpec>
<setSpec>collection:UR1-UFR-SVE</setSpec>
<setSpec>collection:UNIV-AG</setSpec>
<setSpec>collection:IFR140</setSpec>
<setSpec>collection:UR1-HAL</setSpec>
<setSpec>collection:EHESP</setSpec>
<setSpec>collection:USPC</setSpec>
<setSpec>collection:UR1-SDV</setSpec>
<setSpec>collection:UNIV-ANGERS</setSpec>
<setSpec>collection:IRSET-EHESP</setSpec>
</header>
<metadata><dc>
<publisher>HAL CCSD</publisher>
<title lang=en>Staphylococcus aureus alters cell cycle in human and bovine epithelial cells.</title>
<creator>Alekseeva, Ludmila</creator>
<creator>Péton, Vincent</creator>
<creator>ALMEIDA DA SILVA, SINTIA</creator>
<creator>Cousin, Fabien</creator>
<creator>Legembre , Patrick</creator>
<creator>Edmond , Valérie</creator>
<creator>Azevedo, Vasco</creator>
<creator>Miyoshi, Anderson</creator>
<creator>Even, Sergine</creator>
<creator>Taieb, Frederic</creator>
<creator>Arlot-Bonnemains, Yannick</creator>
<creator>Le Loir, Yves</creator>
<creator>Berkova, Nadejda</creator>
<contributor>IBCH, RAS ; Shemyakin-Ovchinnikov Institute of Bio-organic Chemistry</contributor>
<contributor>Science et Technologie du Lait et de l'Oeuf (STLO) ; Institut National de la Recherche Agronomique (INRA) - AGROCAMPUS OUEST</contributor>
<contributor>CEP 31.270-901 ; UFMG, ICB, Depto de Biologica Geral</contributor>
<contributor>Institut de recherche, santé, environnement et travail [Rennes] (Irset) ; Université d'Angers (UA) - Université des Antilles et de la Guyane (UAG) - Université de Rennes 1 (UR1) - École des Hautes Études en Santé Publique [EHESP] (EHESP) - Institut National de la Santé et de la Recherche Médicale (INSERM) - Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique )</contributor>
<contributor>CEP 31.270-901 ; UFMG, ICB, Depto de Biologica Geral</contributor>
<contributor>USC 1043 ; Institut National de la Recherche Agronomique (INRA)</contributor>
<contributor>Institut de Génétique et Développement de Rennes (IGDR) ; Université de Rennes 1 (UR1) - Centre National de la Recherche Scientifique (CNRS) - Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique )</contributor>
<contributor>Université de Rennes 1 (UR1)</contributor>
<source>journées des Microbiologistes de l' INRA 2012</source>
<coverage>L'Isle sur la Sorgue, France</coverage>
<identifier>hal-01209365</identifier>
<identifier>https://hal.archives-ouvertes.fr/hal-01209365</identifier>
<source>https://hal.archives-ouvertes.fr/hal-01209365</source>
<source>journées des Microbiologistes de l' INRA 2012, Nov 2012, L'Isle sur la Sorgue, France. 2012</source>
<identifier>PRODINRA : 174569</identifier>
<language>en</language>
<subject lang=en>staphylococcus aureus</subject>
<subject lang=en>cellule épithéliale</subject>
<subject lang=en>infection</subject>
<subject lang=en>interaction</subject>
<subject>[SDV.IDA] Life Sciences [q-bio]/Food engineering</subject>
<subject>[SDV.AEN] Life Sciences [q-bio]/Food and Nutrition</subject>
<subject>[SDV.MP] Life Sciences [q-bio]/Microbiology and Parasitology</subject>
<type>info:eu-repo/semantics/conferenceObject</type>
<type>Poster communications</type>
<description lang=en>Staphylococcus aureus can cause a wide range of infections in humans and warm-blooded animals. The epithelial surface is the principal site of staphylococcal infection. The goal of this work was to investigate the cytopathic effect of S. aureus and its ability to affect host cell cycle. Cultures of human cervix cancer HeLa and mammary gland bovine MAC-T epithelial cells were used to study in vitro the effects of S. aureus strains from human or ruminant origins. Using microscopic examination, we found that S. aureus induced an enlargement of the host cells and we observed that S. aureus slowed down cell proliferation. Similar effect was observed on both human and bovine cells using the human or the ruminant strains. Microscopic analysis of DAPI-stained cells revealed huge decrease of mitotic indexes in infected HeLa and Mac-T cells, synchronized by Double Thymidine Block. Flow cytometry analysis showed that S. aureus induces G2/M phase delay in synchronous HeLa cells. Western blot analysis revealed that the G2/M phase delay was associated with an accumulation of inactive phosphorylated cyclin-dependent kinase Cdk1/cdc2, a key inducer of mitosis entry, and dephosphorylation of core histone H3, an important component of the epigenetic landscape. Altogether, these results suggest that the delay in G2/M phase transition of the infected epithelial cells may be one of the mechanisms employed by S. aureus to survive and to propagate inside the host. Further investigations are ongoing to fully understand the mechanisms associated to this type of S. aureushost cell interactions.</description>
<date>2012-11-13</date>
</dc>
</metadata>
</record>
</GetRecord>
</OAI-PMH>
