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<datestamp>2018-01-11</datestamp>
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<title lang=en>Symbiodinium isolation by NaOH treatment</title>
<creator>Zamoum, Thamilla</creator>
<creator>Furla, Paola</creator>
<contributor>Symbiose Marine (SM) ; Systématique, adaptation, évolution (SAE) ; Université Pierre et Marie Curie - Paris 6 (UPMC) - Centre National de la Recherche Scientifique (CNRS) - Université Pierre et Marie Curie - Paris 6 (UPMC) - Centre National de la Recherche Scientifique (CNRS) - Evolution Paris Seine ; Université Nice Sophia Antipolis (UNS) ; Université Côte d'Azur (UCA) - Université Côte d'Azur (UCA) - Centre National de la Recherche Scientifique (CNRS) - Université des Antilles et de la Guyane (UAG) - Université Pierre et Marie Curie - Paris 6 (UPMC) - Université Nice Sophia Antipolis (UNS) ; Université Côte d'Azur (UCA) - Université Côte d'Azur (UCA) - Université des Antilles et de la Guyane (UAG)</contributor>
<description>International audience</description>
<source>Journal of Experimental Biology</source>
<identifier>hal-01546180</identifier>
<identifier>https://hal.archives-ouvertes.fr/hal-01546180</identifier>
<source>https://hal.archives-ouvertes.fr/hal-01546180</source>
<source>Journal of Experimental Biology, 2012, 215 (22), pp.3875-3880. 〈10.1242/jeb.074955〉</source>
<identifier>DOI : 10.1242/jeb.074955</identifier>
<relation>info:eu-repo/semantics/altIdentifier/doi/10.1242/jeb.074955</relation>
<language>en</language>
<subject lang=en>zooxanthella density</subject>
<subject lang=en> symbiosis</subject>
<subject lang=en> cnidarian</subject>
<subject lang=en> nucleic acid amplification</subject>
<subject>[SDV.BID.EVO] Life Sciences [q-bio]/Biodiversity/Populations and Evolution [q-bio.PE]</subject>
<type>info:eu-repo/semantics/article</type>
<type>Journal articles</type>
<description lang=en>The presence of photosynthetic zooxanthellae (dinoflagellates) in the tissue of many cnidarians is the main reason for their ecological success (i.e. coral reefs). It could also be the main cause of their demise, as the worldwide bleaching of reef-building coral is nothing less than the breakdown of this symbiotic association. The stability of this relationship is the principal marker for the biomonitoring of cnidarian health. We have therefore developed a new, simple method to isolate zooxanthellae in a few steps using NaOH solution. The protocol was validated in three symbiotic cnidarian species: a sea anemone, a gorgonian and a coral. Our method allows the isolation of intact and viable zooxanthellae with better yields than classic methods, especially for species with a calcareous skeleton. Moreover, the isolated zooxanthellae were free of host nucleic contaminants, facilitating subsequent specific molecular analyses.</description>
<date>2012-11</date>
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