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<datestamp>2017-12-21</datestamp>
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<title lang=en>Ubc9 interacts with Lu/BCAM adhesion glycoproteins and regulates their stability at the membrane of polarized MDCK cells</title>
<creator>Collec, Emmanuel</creator>
<creator>El Nemer, Wassim</creator>
<creator>Gauthier, Emilie</creator>
<creator>Gane, Pierre</creator>
<creator>Lecomte, Marie-Christine</creator>
<creator>Dhermy, Didier</creator>
<creator>Cartron, Jean Pierre</creator>
<creator>Colin, Yves</creator>
<creator>Le Van Kim, Caroline</creator>
<creator>Rahuel, Cécile</creator>
<contributor>Protéines de la membrane érythrocytaire et homologues non-érythroides ; Université des Antilles et de la Guyane (UAG) - Institut National de la Transfusion Sanguine [Paris] (INTS) - Université Paris Diderot - Paris 7 (UPD7) - Université de la Réunion (UR) - Institut National de la Santé et de la Recherche Médicale (INSERM)</contributor>
<description>International audience</description>
<source>ISSN: 0264-6021</source>
<source>EISSN: 1470-8728</source>
<source>Biochemical Journal</source>
<publisher>Portland Press</publisher>
<identifier>hal-00478614</identifier>
<identifier>https://hal.archives-ouvertes.fr/hal-00478614</identifier>
<identifier>https://hal.archives-ouvertes.fr/hal-00478614/document</identifier>
<identifier>https://hal.archives-ouvertes.fr/hal-00478614/file/PEER_stage2_10.1042%252FBJ20060861.pdf</identifier>
<source>https://hal.archives-ouvertes.fr/hal-00478614</source>
<source>Biochemical Journal, Portland Press, 2006, 402 (2), pp.311-319. 〈10.1042/BJ20060861〉</source>
<identifier>DOI : 10.1042/BJ20060861</identifier>
<relation>info:eu-repo/semantics/altIdentifier/doi/10.1042/BJ20060861</relation>
<language>en</language>
<subject lang=fr>Life Sciences</subject>
<type>info:eu-repo/semantics/article</type>
<type>Journal articles</type>
<description lang=en>Lutheran (Lu) blood group and Basal Cell Adhesion Molecule (BCAM) antigens both reside on two glycoprotein (gp) isoforms, Lu and Lu(v13), that differ by the size of their cytoplasmic tail. They are receptors of laminin-10/11 and are expressed in red blood cells (RBCs), epithelial cells of multiple tissues and vascular endothelial cells. To gain more insights into the biological function of Lu/BCAM gps, we looked for potential partners of their cytoplasmic tail. We isolated Ubc9 (Ubiquitin-conjugating enzyme 9) protein by screening a human kidney library using the yeast two-hybrid system. Lu/Ubc9 interaction was validated by GST pull-down and co-immunoprecipitation experiments. Endogenous Ubc9 formed a complex with endogenous or recombinant Lu gp in A498 and MDCK epithelial cells, respectively. Substitution of lysine 585 by alanine in the Lu gp, abolished in vitro and ex vivo interaction of Lu gp with Ubc9 protein. Lu K585A mutant transfected in MDCK cells exhibited a normal basolateral membrane expression but was overexpressed at the surface of polarized MDCK cells as compared to wild-type Lu. Pulse-chase experiments showed extended half-life of Lu K585A gp at the plasma membrane, suggesting an impaired endocytosis of this mutant leading to protein accumulation at the membrane. Furthermore, we showed that the ability of MDCK-Lu K585A cells to spread on immobilized laminin was dramatically decreased. Our data supports a physiological role for the direct interaction between Lu gp and Ubc9 protein, and reveals a role for this enzyme in regulating the stability of Lu gp at the cell membrane.</description>
<date>2006-11-06</date>
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