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<title lang=en>Are Niemann-Pick type C proteins key players in cnidarian-dinoflagellate endosymbioses?</title>
<creator>Dani, Vincent</creator>
<creator>Ganot, Philippe</creator>
<creator>Priouzeau, Fabrice</creator>
<creator>Furla, Paola</creator>
<creator>Sabourault, Cecile</creator>
<contributor>Symbiose Marine (SM) ; Systématique, adaptation, évolution (SAE) ; Université Pierre et Marie Curie - Paris 6 (UPMC) - Centre National de la Recherche Scientifique (CNRS) - Université Pierre et Marie Curie - Paris 6 (UPMC) - Centre National de la Recherche Scientifique (CNRS) - Evolution Paris Seine ; Université Nice Sophia Antipolis (UNS) ; Université Côte d'Azur (UCA) - Université Côte d'Azur (UCA) - Centre National de la Recherche Scientifique (CNRS) - Université des Antilles et de la Guyane (UAG) - Université Pierre et Marie Curie - Paris 6 (UPMC) - Université Nice Sophia Antipolis (UNS) ; Université Côte d'Azur (UCA) - Université Côte d'Azur (UCA) - Université des Antilles et de la Guyane (UAG)</contributor>
<contributor>French ANR [ANR-12-JSV7-0009-01]</contributor>
<contributor> MESR/University of Nice</contributor>
<description>International audience</description>
<source>ISSN: 0962-1083</source>
<source>EISSN: 1365-294X</source>
<source>Molecular Ecology</source>
<publisher>Wiley</publisher>
<identifier>hal-01546166</identifier>
<identifier>https://hal.archives-ouvertes.fr/hal-01546166</identifier>
<source>https://hal.archives-ouvertes.fr/hal-01546166</source>
<source>Molecular Ecology, Wiley, 2014, 23 (18), pp.4527-4540. 〈10.1111/mec.12876〉</source>
<identifier>DOI : 10.1111/mec.12876</identifier>
<relation>info:eu-repo/semantics/altIdentifier/doi/10.1111/mec.12876</relation>
<language>en</language>
<subject lang=en>bleaching</subject>
<subject lang=en> cnidarian-dinoflagellate interaction</subject>
<subject lang=en> NPC1</subject>
<subject lang=en> NPC2</subject>
<subject lang=en> sterol transport</subject>
<subject lang=en> symbiosis</subject>
<subject>[SDV.BID.EVO] Life Sciences [q-bio]/Biodiversity/Populations and Evolution [q-bio.PE]</subject>
<type>info:eu-repo/semantics/article</type>
<type>Journal articles</type>
<description lang=en>The symbiotic interaction between cnidarians, such as corals and sea anemones, and the unicellular algae Symbiodinium is regulated by yet poorly understood cellular mechanisms, despite the ecological importance of coral reefs. These mechanisms, including host-symbiont recognition and metabolic exchange, control symbiosis stability under normal conditions, but also lead to symbiosis breakdown (bleaching) during stress. This study describes the repertoire of the sterol-trafficking proteins Niemann-Pick type C (NPC1 and NPC2) in the symbiotic sea anemone Anemonia viridis. We found one NPC1 gene in contrast to the two genes (NPC1 and NPC1L1) present in vertebrate genomes. While only one NPC2 gene is present in many metazoans, this gene has been duplicated in cnidarians, and we detected four NPC2 genes in A. viridis. However, only one gene (AvNPC2-d) was upregulated in symbiotic relative to aposymbiotic sea anemones and displayed higher expression in the gastrodermis (symbiont-containing tissue) than in the epidermis. We performed immunolabelling experiments on tentacle cross sections and demonstrated that the AvNPC2-d protein was closely associated with symbiosomes. In addition, AvNPC1 and AvNPC2-d gene expression was strongly downregulated during stress. These data suggest that AvNPC2-d is involved in both the stability and dysfunction of cnidarian-dinoflagellate symbioses.</description>
<date>2014-09</date>
</dc>
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