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<identifier>oai:HAL:hal-00828987v1</identifier>
<datestamp>2014-10-13</datestamp>
<setSpec>type:ART</setSpec>
<setSpec>subject:chim</setSpec>
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<publisher>HAL CCSD</publisher>
<title lang=en>Assessment of poly-L-lysine dendrigrafts for virus concentration in water: use of MS2 bacteriophage as proof of concept.</title>
<creator>Cadiere, Axelle</creator>
<creator>Couturaud, Benoit</creator>
<creator>Boismard, Jeanne</creator>
<creator>Le Cann, Pierre</creator>
<creator>Gerard, Anne</creator>
<creator>Mas, André</creator>
<creator>Faye, Clément</creator>
<creator>Garelli, Laurent</creator>
<creator>Roig, Bernoit</creator>
<contributor>LERES ; Institut de recherche, santé, environnement et travail [Rennes] (Irset) ; INSERM - École Nationale de la Santé Publique - Université de Rennes 1 (UR1) - Université des Antilles et de la Guyane (UAG) - Structure Fédérative de Recherche en Biologie-Santé de Rennes (Biosit) ; Université de Rennes 1 (UR1) - INSERM - CNRS - INSERM - CNRS - INSERM - École Nationale de la Santé Publique - Université de Rennes 1 (UR1) - Université des Antilles et de la Guyane (UAG) - Structure Fédérative de Recherche en Biologie-Santé de Rennes (Biosit) ; Université de Rennes 1 (UR1) - INSERM - CNRS - INSERM - CNRS - École des hautes études en santé publique [Rennes] (EHESP) ; Université européenne de Bretagne (UEB) - PRES Sorbonne Paris Cité - Université européenne de Bretagne (UEB) - PRES Sorbonne Paris Cité</contributor>
<contributor>Institut Charles Gerhardt - Institut de Chimie Moléculaire et des Matériaux de Montpellier (ICG ICMMM) ; CNRS - Université Montpellier 1 (UM1) - Université Montpellier 2 - Sciences et Techniques (UM2) - Ecole Nationale Supérieure de Chimie de Montpellier</contributor>
<contributor>École des hautes études en santé publique [Rennes] (EHESP) ; Université européenne de Bretagne (UEB) - PRES Sorbonne Paris Cité</contributor>
<contributor>Institut des Biomolécules Max Mousseron (IBMM) ; CNRS - Université Montpellier 1 (UM1) - Université Montpellier 2 - Sciences et Techniques (UM2)</contributor>
<contributor>Colcom SARL ; Colcom SARL</contributor>
<description>International audience</description>
<source>Journal of Applied Microbiology</source>
<publisher>Wiley-Blackwell</publisher>
<identifier>hal-00828987</identifier>
<identifier>https://hal.archives-ouvertes.fr/hal-00828987</identifier>
<source>https://hal.archives-ouvertes.fr/hal-00828987</source>
<source>Journal of Applied Microbiology, Wiley-Blackwell, 2013, 115 (1), pp.290-7. <10.1111/jam.12209></source>
<identifier>DOI : 10.1111/jam.12209</identifier>
<identifier>PUBMED : 23551794</identifier>
<language>en</language>
<subject lang=en>concentration</subject>
<subject lang=en>diagnosis</subject>
<subject lang=en>poly-l-lysine dendrigrafts</subject>
<subject lang=en>virus</subject>
<subject lang=en>water safety</subject>
<subject>[CHIM.POLY] Chemical Sciences/Polymers</subject>
<subject>[SDV.MP] Life Sciences/Microbiology and Parasitology</subject>
<type>Journal articles</type>
<description lang=en>AIMS: Virus detection has often been difficult due to a low concentration in water. In this study, we developed a new procedure based on concentration of virus particles on an innovative support: poly-L-lysine dendrigrafts (DGL), coupled with directed nucleic acid extraction and real-time PCR quantification. METHODS AND RESULTS: This method was evaluated using the bacteriophage MS2 as a model virus. This virus exhibited the size and structural properties of human pathogenic enteric viruses and has often been used to assess new supports of concentration. Moreover, this bacteriophage is also a faecal contamination indicator. In this study, many water filtration conditions were tested (volume of water, concentration, etc.), and more than 80% of bacteriophage were recovered after filtration on polymer, in most conditions. We demonstrated that the method was linear (slope = 0*99 ± 0*04 and Y intercept when x = -0*02 ± 0*28), valid (as manipulators, tested concentrations, volumes of sample and batch of polymer did not have any influence on concentration) and sensitive (allowing to concentrate up to 16,600-fold 1 l of sample and to detect and quantify down to 750 GC l(-1) and 7500 GC l(-1), respectively). CONCLUSIONS: To conclude, this support exhibits high interest to retain viruses and to allow to detect low concentration of virus in water. SIGNIFICANCE AND IMPACT OF THE STUDY: This study gives valuable advance in the methods of concentration and diagnosis of virus in water.</description>
<date>2013-07</date>
</dc>
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