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<datestamp>2017-12-21</datestamp>
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<title lang=en>Quantitative proteomic Isotope-Coded Protein Label (ICPL) analysis reveals alteration of several functional processes in the glioblastoma.</title>
<creator>Com, Emmanuelle</creator>
<creator>Clavreul, Anne</creator>
<creator>Lagarrigue, Mélanie</creator>
<creator>Michalak, Sophie</creator>
<creator>Menei, Philippe</creator>
<creator>Pineau, Charles</creator>
<contributor>Plateforme Protéomique-Biogenouest (PPB) ; Institut de recherche, santé, environnement et travail [Rennes] (Irset) ; Université d'Angers (UA) - Université des Antilles et de la Guyane (UAG) - Université de Rennes 1 (UR1) - École des Hautes Études en Santé Publique [EHESP] (EHESP) - Institut National de la Santé et de la Recherche Médicale (INSERM) - Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ) - Université d'Angers (UA) - Université des Antilles et de la Guyane (UAG) - Université de Rennes 1 (UR1) - École des Hautes Études en Santé Publique [EHESP] (EHESP) - Institut National de la Santé et de la Recherche Médicale (INSERM) - Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ) - Proteomics Core Facility (Protim) ; Université de Rennes 1 (UR1) - Plateforme Génomique Santé Biogenouest® - Plateforme Génomique Santé Biogenouest®</contributor>
<contributor>Ingénierie de la vectorisation particulaire ; Université d'Angers (UA) - Institut National de la Santé et de la Recherche Médicale (INSERM)</contributor>
<contributor>Laboratoire de Pathologie Cellulaire et Tissulaire ; CHU Angers</contributor>
<contributor>Biogenouest; French National Cancer Institute (INCa); IBiSA; Conseil Régional de Bretagne grants</contributor>
<description>International audience</description>
<source>ISSN: 1874-3919</source>
<source>EISSN: 1876-7737</source>
<source>Journal of Proteomics</source>
<publisher>Elsevier</publisher>
<identifier>hal-00877756</identifier>
<identifier>https://hal.archives-ouvertes.fr/hal-00877756</identifier>
<source>https://hal.archives-ouvertes.fr/hal-00877756</source>
<source>Journal of Proteomics, Elsevier, 2012, 75 (13), pp.3898-913. 〈10.1016/j.jprot.2012.04.034〉</source>
<identifier>DOI : 10.1016/j.jprot.2012.04.034</identifier>
<relation>info:eu-repo/semantics/altIdentifier/doi/10.1016/j.jprot.2012.04.034</relation>
<identifier>PUBMED : 22575386</identifier>
<relation>info:eu-repo/semantics/altIdentifier/pmid/22575386</relation>
<language>en</language>
<subject lang=it>Proteome</subject>
<subject lang=it>Glioblastoma</subject>
<subject lang=it>Cancer</subject>
<subject lang=it>ICPL</subject>
<subject>[SDV.BBM] Life Sciences [q-bio]/Biochemistry, Molecular Biology</subject>
<type>info:eu-repo/semantics/article</type>
<type>Journal articles</type>
<description lang=en>Glioblastoma (GB), the most frequent primary tumor of the central nervous system, remains one of the most lethal human cancers despite intensive researches. Current paradigm in the study of GB has been focused on inter-patient variability and on trying to isolate new classification elements or prognostic factors. Here, using ICPL, a technique for protein relative quantification by mass spectrometry, we investigated protein expression between the four regions of GB on clinically relevant biopsies from 5 patients. We identified 584 non-redundant proteins and 31 proteins were found to be up-regulated in the tumor region compared to the peri-tumoral brain tissue, among which, 24 proteins belong to an interaction network linked to 4 biological processes. The core of this network is mainly constituted of interactions between beta-actin (ACTB) with heat shock proteins (HSP90AA1, HSPA8) and 14-3-3 proteins (YWHAZ, YWHAG, YWHAB). A cluster of three isoforms of the sodium pump α-subunit (ATP1A1, ATP1A2, ATP1A3) was also identified outside this network. The differential expression observed for ACTB and 14-3-3γ was further validated by western blot and/or immunohistochemistry. Our study confirms the identity of previously proposed molecular targets, highlights several functional processes altered in GB such as energy metabolism and synaptic transmission and could thus provide added value to new therapeutic trails.</description>
<date>2012-07-16</date>
</dc>
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