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<publisher>HAL CCSD</publisher>
<title lang=en>Screening Estrogenic Activities of Chemicals or Mixtures In Vivo Using Transgenic (cyp19a1b-GFP) Zebrafish Embryos</title>
<creator>Brion, François</creator>
<creator>Le Page, Yann</creator>
<creator>Piccini, Benjamin</creator>
<creator>Cardoso, Olivier</creator>
<creator>Tong, Sok-Keng</creator>
<creator>Chung, Bon-Chu</creator>
<creator>Kah, Olivier</creator>
<contributor>Institut National de l'Environnement Industriel et des Risques (INERIS)</contributor>
<contributor>Institut de recherche, santé, environnement et travail [Rennes] (Irset) ; Université d'Angers (UA) - Université des Antilles et de la Guyane (UAG) - Université de Rennes 1 (UR1) - École des Hautes Études en Santé Publique [EHESP] (EHESP) - Institut National de la Santé et de la Recherche Médicale (INSERM) - Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique )</contributor>
<contributor>Institute of Molecular Biology (IMB Sinica) ; Academia Sinica</contributor>
<description>International audience</description>
<source>ISSN: 1932-6203</source>
<source>PLoS ONE</source>
<publisher>Public Library of Science</publisher>
<identifier>hal-00877371</identifier>
<identifier>https://hal.archives-ouvertes.fr/hal-00877371</identifier>
<identifier>https://hal.archives-ouvertes.fr/hal-00877371/document</identifier>
<identifier>https://hal.archives-ouvertes.fr/hal-00877371/file/Screening_Estrogenic_Activities-accepted.pdf</identifier>
<source>https://hal.archives-ouvertes.fr/hal-00877371</source>
<source>PLoS ONE, Public Library of Science, 2012, 7 (5), pp.e36069. 〈10.1371/journal.pone.0036069〉</source>
<identifier>DOI : 10.1371/journal.pone.0036069</identifier>
<relation>info:eu-repo/semantics/altIdentifier/doi/10.1371/journal.pone.0036069</relation>
<identifier>PUBMED : 22586461</identifier>
<relation>info:eu-repo/semantics/altIdentifier/pmid/22586461</relation>
<language>en</language>
<subject>[SDV.BDLR] Life Sciences [q-bio]/Reproductive Biology</subject>
<type>info:eu-repo/semantics/article</type>
<type>Journal articles</type>
<description lang=en>The tg(cyp19a1b-GFP) transgenic zebrafish expresses GFP (green fluorescent protein) under the control of the cyp19a1b gene, encoding brain aromatase. This gene has two major characteristics: (i) it is only expressed in radial glial progenitors in the brain of fish and (ii) it is exquisitely sensitive to estrogens. Based on these properties, we demonstrate that natural or synthetic hormones (alone or in binary mixture), including androgens or progestagens, and industrial chemicals induce a concentration-dependent GFP expression in radial glial progenitors. As GFP expression can be quantified by in vivo imaging, this model presents a very powerful tool to screen and characterize compounds potentially acting as estrogen mimics either directly or after metabolization by the zebrafish embryo. This study also shows that radial glial cells that act as stem cells are direct targets for a large panel of endocrine disruptors, calling for more attention regarding the impact of environmental estrogens and/or certain pharmaceuticals on brain development. Altogether these data identify this in vivo bioassay as an interesting alternative to detect estrogen mimics in hazard and risk assessment perspective.</description>
<date>2012</date>
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