untitled
<OAI-PMH schemaLocation=http://www.openarchives.org/OAI/2.0/ http://www.openarchives.org/OAI/2.0/OAI-PMH.xsd>
<responseDate>2018-01-15T18:29:53Z</responseDate>
<request identifier=oai:HAL:hal-01134174v1 verb=GetRecord metadataPrefix=oai_dc>http://api.archives-ouvertes.fr/oai/hal/</request>
<GetRecord>
<record>
<header>
<identifier>oai:HAL:hal-01134174v1</identifier>
<datestamp>2018-01-11</datestamp>
<setSpec>type:ART</setSpec>
<setSpec>subject:sdv</setSpec>
<setSpec>collection:UNIV-RENNES1</setSpec>
<setSpec>collection:CNRS</setSpec>
<setSpec>collection:UNIV-AG</setSpec>
<setSpec>collection:IRSET</setSpec>
<setSpec>collection:IPR</setSpec>
<setSpec>collection:IPR-OP</setSpec>
<setSpec>collection:IRSET-NEED</setSpec>
<setSpec>collection:IRSET-SMLF</setSpec>
<setSpec>collection:IFR140</setSpec>
<setSpec>collection:BIOSIT</setSpec>
<setSpec>collection:UR1-SPM</setSpec>
<setSpec>collection:UR1-UFR-SVE</setSpec>
<setSpec>collection:UR1-UFR-SPM</setSpec>
<setSpec>collection:STATS-UR1</setSpec>
<setSpec>collection:UR1-SDLM</setSpec>
<setSpec>collection:UR1-SDLMJONCH</setSpec>
<setSpec>collection:UR1-SDV</setSpec>
<setSpec>collection:UR1-HAL</setSpec>
<setSpec>collection:EHESP</setSpec>
<setSpec>collection:USPC</setSpec>
<setSpec>collection:IRSET-5</setSpec>
<setSpec>collection:IRSET-6</setSpec>
<setSpec>collection:UNIV-ANGERS</setSpec>
<setSpec>collection:OSUR</setSpec>
<setSpec>collection:IPR-MM</setSpec>
</header>
<metadata><dc>
<publisher>HAL CCSD</publisher>
<title lang=en>Structural origin of the drastic modification of second harmonic generation intensity pattern occurring in tail muscles of climax stages xenopus tadpoles.</title>
<creator>Recher, Gaëlle</creator>
<creator>Coumailleau, Pascal</creator>
<creator>Rouède, Denis</creator>
<creator>Tiaho, François</creator>
<contributor>Institut de recherche, santé, environnement et travail [Rennes] (Irset) ; Université d'Angers (UA) - Université des Antilles et de la Guyane (UAG) - Université de Rennes 1 (UR1) - École des Hautes Études en Santé Publique [EHESP] (EHESP) - Institut National de la Santé et de la Recherche Médicale (INSERM) - Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique )</contributor>
<contributor>Dept of Physiology, Development and Neurosciences, Anatomy building, University of Cambridge ; Anatomy building, University of Cambridge</contributor>
<contributor>Institut de Physique de Rennes (IPR) ; Université de Rennes 1 (UR1) - Centre National de la Recherche Scientifique (CNRS)</contributor>
<contributor>This work was supported by Région Bretagne, Rennes Métropole, Conseil Général d’Ille-et-Villaine, CRITT Santé Bretagne, CBB Developpement Bretagne, Ministère de l’Enseignement Supérieur et de la Recherche and the European Union Federal Funds FEDER.</contributor>
<description>International audience</description>
<source>ISSN: 1047-8477</source>
<source>EISSN: 1095-8657</source>
<source>Journal of Structural Biology</source>
<publisher>Elsevier</publisher>
<identifier>hal-01134174</identifier>
<identifier>https://hal-univ-rennes1.archives-ouvertes.fr/hal-01134174</identifier>
<identifier>https://hal-univ-rennes1.archives-ouvertes.fr/hal-01134174/document</identifier>
<identifier>https://hal-univ-rennes1.archives-ouvertes.fr/hal-01134174/file/Recher%20et%20al_JSB-2015_hal.pdf</identifier>
<source>https://hal-univ-rennes1.archives-ouvertes.fr/hal-01134174</source>
<source>Journal of Structural Biology, Elsevier, 2015, 190 (1), pp.1-10. 〈10.1016/j.jsb.2015.03.003〉</source>
<identifier>DOI : 10.1016/j.jsb.2015.03.003</identifier>
<relation>info:eu-repo/semantics/altIdentifier/doi/10.1016/j.jsb.2015.03.003</relation>
<identifier>PUBMED : 25770062</identifier>
<relation>info:eu-repo/semantics/altIdentifier/pmid/25770062</relation>
<language>en</language>
<subject lang=en>Skeletal muscle</subject>
<subject lang=en>Second harmonic generation microscopy</subject>
<subject lang=en>Two photon excitation fluorescence</subject>
<subject lang=en>Electron microscopy</subject>
<subject lang=en>Xenopus</subject>
<subject lang=en>Apoptosis</subject>
<subject>[SDV] Life Sciences [q-bio]</subject>
<type>info:eu-repo/semantics/article</type>
<type>Journal articles</type>
<description lang=en>Second harmonic generation (SHG) microscopy is a powerful tool for studying submicron architecture of muscles tissues. Using this technique, we show that the canonical single frequency sarcomeric SHG intensity pattern (SHG-IP) of premetamorphic xenopus tadpole tail muscles is converted to double frequency (2f) sarcomeric SHG-IP in metamorphic climax stages due to massive physiological muscle proteolysis. This conversion was found to rise from 7% in premetamorphic muscles to about 97% in fragmented muscular apoptotic bodies. Moreover a 66% conversion was also found in non-fragmented metamorphic tail muscles. Also, a strong correlation between predominant 2f sarcomeric SHG-IPs and myofibrillar misalignment is established with electron microscopy. Experimental and theoretical results demonstrate the higher sensitivity and the supra resolution power of SHG microscopy over TPEF to reveal 3D myofibrillar misalignment. From this study, we suggest that 2f sarcomeric SHG-IP could be used as signature of triad defect and disruption of excitation–contraction coupling. As the mechanism of muscle proteolysis is similar to that found in mdx mouse muscles, we further suggest that xenopus tadpole tail resorption at climax stages could be used as an alternative or complementary model of Duchene muscular dystrophy.</description>
<date>2015-03-11</date>
</dc>
</metadata>
</record>
</GetRecord>
</OAI-PMH>
