untitled
<OAI-PMH schemaLocation=http://www.openarchives.org/OAI/2.0/ http://www.openarchives.org/OAI/2.0/OAI-PMH.xsd>
<responseDate>2018-01-15T18:23:00Z</responseDate>
<request identifier=oai:HAL:hal-01327354v1 verb=GetRecord metadataPrefix=oai_dc>http://api.archives-ouvertes.fr/oai/hal/</request>
<GetRecord>
<record>
<header>
<identifier>oai:HAL:hal-01327354v1</identifier>
<datestamp>2018-01-12</datestamp>
<setSpec>type:ART</setSpec>
<setSpec>subject:sdv</setSpec>
<setSpec>collection:UPMC</setSpec>
<setSpec>collection:CNRS</setSpec>
<setSpec>collection:UNIV-AG</setSpec>
<setSpec>collection:UNICE</setSpec>
<setSpec>collection:EVOLUTION_PARIS_SEINE</setSpec>
<setSpec>collection:CEA</setSpec>
<setSpec>collection:DSV</setSpec>
<setSpec>collection:CEA-UPSAY</setSpec>
<setSpec>collection:I2BC</setSpec>
<setSpec>collection:PASTEUR</setSpec>
<setSpec>collection:UNIV-PSUD</setSpec>
<setSpec>collection:UNIV-PSUD-SACLAY</setSpec>
<setSpec>collection:CEA-UPSAY-SACLAY</setSpec>
<setSpec>collection:UNIV-PARIS-SACLAY</setSpec>
<setSpec>collection:UCA-TEST</setSpec>
<setSpec>collection:UPMC_POLE_4</setSpec>
<setSpec>collection:IBPS</setSpec>
<setSpec>collection:EVOL_PARIS_SEINE-GGT</setSpec>
<setSpec>collection:UNIV-COTEDAZUR</setSpec>
</header>
<metadata><dc>
<publisher>HAL CCSD</publisher>
<title lang=en>topIb, a phylogenetic hallmark gene of Thaumarchaeota encodes a functional eukaryote-like topoisomerase IB</title>
<creator>Dahmane, Narimane</creator>
<creator>Gadelle, Danièle</creator>
<creator>Delmas, Stéphane</creator>
<creator>Criscuolo, Alexis</creator>
<creator>Eberhard, Stephan</creator>
<creator>Desnoues, Nicole</creator>
<creator>Collin, Sylvie</creator>
<creator>Zhang, Hongliang</creator>
<creator>Pommier, Yves</creator>
<creator>Forterre, Patrick</creator>
<creator>Sezonov, Guennadi</creator>
<contributor>Evolution Paris Seine ; Université Nice Sophia Antipolis (UNS) ; Université Côte d'Azur (UCA) - Université Côte d'Azur (UCA) - Centre National de la Recherche Scientifique (CNRS) - Université des Antilles et de la Guyane (UAG) - Université Pierre et Marie Curie - Paris 6 (UPMC)</contributor>
<contributor>Institut de Biologie Intégrative de la Cellule (I2BC) ; Université Paris-Sud - Paris 11 (UP11) - Commissariat à l'énergie atomique et aux énergies alternatives (CEA) - Université Paris-Saclay - Centre National de la Recherche Scientifique (CNRS)</contributor>
<contributor>Centre d'Informatique pour la Biologie ; Institut Pasteur [Paris]</contributor>
<contributor>Biologie Moléculaire du Gène chez les Extrêmophiles (BMGE) ; Institut Pasteur [Paris]</contributor>
<contributor>National Cancer Institute, National Institutes of Health, Bethesda</contributor>
<description>International audience</description>
<source>ISSN: 0305-1048</source>
<source>EISSN: 1362-4962</source>
<source>Nucleic Acids Research</source>
<publisher>Oxford University Press</publisher>
<identifier>hal-01327354</identifier>
<identifier>http://hal.upmc.fr/hal-01327354</identifier>
<identifier>http://hal.upmc.fr/hal-01327354/document</identifier>
<identifier>http://hal.upmc.fr/hal-01327354/file/Nucl.%20Acids%20Res.-2016-Dahmane-2795-805.pdf</identifier>
<source>http://hal.upmc.fr/hal-01327354</source>
<source>Nucleic Acids Research, Oxford University Press, 2016, 44 (6), pp.2795-2805. 〈10.1093/nar/gkw097〉</source>
<identifier>DOI : 10.1093/nar/gkw097</identifier>
<relation>info:eu-repo/semantics/altIdentifier/doi/10.1093/nar/gkw097</relation>
<language>en</language>
<subject>[SDV.BBM] Life Sciences [q-bio]/Biochemistry, Molecular Biology</subject>
<type>info:eu-repo/semantics/article</type>
<type>Journal articles</type>
<description lang=en>Type IB DNA topoisomerases can eliminate torsional stresses produced during replication and transcription. These enzymes are found in all eukaryotes and a short version is present in some bacteria and viruses. Among prokaryotes, the long eukaryotic version is only observed in archaea of the phylum Thau-marchaeota. However, the activities and the roles of these topoisomerases have remained an open question. Here, we demonstrate that all available thaumar-chaeal genomes contain a topoisomerase IB gene that defines a monophyletic group closely related to the eukaryotic enzymes. We show that the topIB gene is expressed in the model thaumarchaeon Ni-trososphaera viennensis and we purified the recom-binant enzyme from the uncultivated thaumarchaeon Candidatus Caldiarchaeum subterraneum. This enzyme is active in vitro at high temperature, making it the first thermophilic topoisomerase IB characterized so far. We have compared this archaeal type IB enzyme to its human mitochondrial and nuclear counterparts. The archaeal enzyme relaxes both negatively and positively supercoiled DNA like the eukaryotic enzymes. However, its pattern of DNA cleavage specificity is different and it is resistant to camptothecins (CPTs) and non-CPT Top1 inhibitors, LMP744 and lamellarin D. This newly described ther-mostable topoisomerases IB should be a promising new model for evolutionary, mechanistic and structural studies.</description>
<rights>http://creativecommons.org/licenses/by/</rights>
<date>2016-04-07</date>
</dc>
</metadata>
</record>
</GetRecord>
</OAI-PMH>
