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<title lang=en>The Disintegrin and Metalloprotease ADAM12 Is Associated with TGF-β-Induced Epithelial to Mesenchymal Transition</title>
<creator>Ruff, Michaël</creator>
<creator>Leyme, Anthony</creator>
<creator>Le Cann, Fabienne</creator>
<creator>Bonnier, Dominique</creator>
<creator>Le Seyec, Jacques</creator>
<creator>Chesnel, Franck</creator>
<creator>Fattet, Laurent</creator>
<creator>Rimokh, Ruth</creator>
<creator>Baffet, Georges</creator>
<creator>Théret, Nathalie</creator>
<contributor>Institut de recherche, santé, environnement et travail [Rennes] (Irset) ; Université d'Angers (UA) - Université des Antilles et de la Guyane (UAG) - Université de Rennes 1 (UR1) - École des Hautes Études en Santé Publique [EHESP] (EHESP) - Institut National de la Santé et de la Recherche Médicale (INSERM) - Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique )</contributor>
<contributor>Institut de Génétique et Développement de Rennes (IGDR) ; Université de Rennes 1 (UR1) - Centre National de la Recherche Scientifique (CNRS) - Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique )</contributor>
<contributor>Centre de Recherche en Cancérologie de Lyon (CRCL) ; Centre Léon Bérard [Lyon] - Université Claude Bernard Lyon 1 (UCBL) - Institut National de la Santé et de la Recherche Médicale (INSERM) - Centre National de la Recherche Scientifique (CNRS)</contributor>
<contributor>Université européenne de Bretagne (UEB)</contributor>
<description>International audience</description>
<source>ISSN: 1932-6203</source>
<source>PLoS ONE</source>
<publisher>Public Library of Science</publisher>
<identifier>hal-01207281</identifier>
<identifier>https://hal-univ-rennes1.archives-ouvertes.fr/hal-01207281</identifier>
<source>https://hal-univ-rennes1.archives-ouvertes.fr/hal-01207281</source>
<source>PLoS ONE, Public Library of Science, 2015, 10 (9), pp.e0139179. 〈10.1371/journal.pone.0139179〉</source>
<identifier>DOI : 10.1371/journal.pone.0139179</identifier>
<relation>info:eu-repo/semantics/altIdentifier/doi/10.1371/journal.pone.0139179</relation>
<identifier>PUBMED : 26407179</identifier>
<relation>info:eu-repo/semantics/altIdentifier/pmid/26407179</relation>
<language>en</language>
<subject>[SDV] Life Sciences [q-bio]</subject>
<type>info:eu-repo/semantics/article</type>
<type>Journal articles</type>
<description lang=en>The increased expression of the Disintegrin and Metalloprotease ADAM12 has been associated with human cancers, however its role remain unclear. We have previously reported that ADAM12 expression is induced by the transforming growth factor, TGF-β and promotes TGF-β-dependent signaling through interaction with the type II receptor of TGF-β. Here we explore the implication of ADAM12 in TGF-β-mediated epithelial to mesenchymal transition (EMT), a key process in cancer progression. We show that ADAM12 expression is correlated with EMT markers in human breast cancer cell lines and biopsies. Using a non-malignant breast epithelial cell line (MCF10A), we demonstrate that TGF-β-induced EMT increases expression of the membrane-anchored ADAM12L long form. Importantly, ADAM12L overexpression in MCF10A is sufficient to induce loss of cell-cell contact, reorganization of actin cytoskeleton, up-regulation of EMT markers and chemoresistance. These effects are independent of the proteolytic activity but require the cytoplasmic tail and are specific of ADAM12L since overexpression of ADAM12S failed to induce similar changes. We further demonstrate that ADAM12L-dependent EMT is associated with increased phosphorylation of Smad3, Akt and ERK proteins. Conversely, inhibition of TGF-β receptors or ERK activities reverses ADAM12L-induced mesenchymal phenotype. Together our data demonstrate that ADAM12L is associated with EMT and contributes to TGF-β-dependent EMT by favoring both Smad-dependent and Smad-independent pathways</description>
<date>2015</date>
</dc>
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