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<title lang=en>Developmental stage-dependent metabolic regulation during meiotic differentiation in budding yeast</title>
<creator>Walther, Thomas</creator>
<creator>Létisse, Fabien</creator>
<creator>Peyriga, Lindsay</creator>
<creator>Alkim, Ceren</creator>
<creator>Liu, Yuchen</creator>
<creator>Lardenois, Aurélie</creator>
<creator>Martin-Yken, Hélène</creator>
<creator>Portais, Jean-Charles</creator>
<creator>Primig, Michael</creator>
<creator>François, Jean-Marie</creator>
<contributor>Laboratoire d'Ingénierie des Systèmes Biologiques et des Procédés (LISBP) ; Institut National de la Recherche Agronomique (INRA) - Institut National des Sciences Appliquées - Toulouse (INSA Toulouse) - Centre National de la Recherche Scientifique (CNRS)</contributor>
<contributor>Transcriptional networks in gametogenesis and cancer ; Institut de recherche, santé, environnement et travail [Rennes] (Irset) ; Université d'Angers (UA) - Université des Antilles et de la Guyane (UAG) - Université de Rennes 1 (UR1) - École des Hautes Études en Santé Publique [EHESP] (EHESP) - Institut National de la Santé et de la Recherche Médicale (INSERM) - Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ) - Université d'Angers (UA) - Université des Antilles et de la Guyane (UAG) - Université de Rennes 1 (UR1) - École des Hautes Études en Santé Publique [EHESP] (EHESP) - Institut National de la Santé et de la Recherche Médicale (INSERM) - Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique )</contributor>
<contributor>This project was supported by a post-doctoral DFG fellowship (WA2163)awarded to TW, an FRM fourth-year PhD fellowship awarded to YL, InsermAvenir grant (R07216NS) and Région Bretagne CREATE grant (R11016NN)awarded to MP, and research grants ANR (NT05-2_448) and BLAN07-2_200101 awarded to JMF.</contributor>
<description>International audience</description>
<source>ISSN: 1471-2180</source>
<source>BMC Microbiology</source>
<publisher>BioMed Central</publisher>
<identifier>inserm-01094132</identifier>
<identifier>http://www.hal.inserm.fr/inserm-01094132</identifier>
<identifier>http://www.hal.inserm.fr/inserm-01094132/document</identifier>
<identifier>http://www.hal.inserm.fr/inserm-01094132/file/s12915-014-0060-x.pdf</identifier>
<source>http://www.hal.inserm.fr/inserm-01094132</source>
<source>BMC Microbiology, BioMed Central, 2014, 139, pp.297. 〈10.1186/s12915-014-0060-x〉</source>
<identifier>DOI : 10.1186/s12915-014-0060-x</identifier>
<relation>info:eu-repo/semantics/altIdentifier/doi/10.1186/s12915-014-0060-x</relation>
<identifier>PUBMED : 25178389</identifier>
<relation>info:eu-repo/semantics/altIdentifier/pmid/25178389</relation>
<language>en</language>
<subject lang=en>Differentiation</subject>
<subject lang=en>Flux analysis</subject>
<subject lang=en>Meiosis</subject>
<subject lang=en>Metabolic reprogramming</subject>
<subject lang=en>Metabolome</subject>
<subject lang=en>Systems biology</subject>
<subject lang=en>Transcriptome</subject>
<subject lang=en>Yeast Background</subject>
<subject>[SDV] Life Sciences [q-bio]</subject>
<type>info:eu-repo/semantics/article</type>
<type>Journal articles</type>
<description lang=en>Background: The meiotic developmental pathway in yeast enables both differentiation of vegetative cells into haploid spores that ensure long-term survival, and recombination of the parental DNA to create genetic diversity. Despite the importance of proper metabolic regulation for the supply of building blocks and energy, little is known about the reprogramming of central metabolic pathways in meiotically differentiating cells during passage through successive developmental stages. Results: Metabolic regulation during meiotic differentiation in budding yeast was analyzed by integrating information on genome-wide transcriptional activity, 26 enzymatic activities in the central metabolism, the dynamics of 67 metabolites, and a metabolic flux analysis at mid-stage meiosis. Analyses of mutants arresting sporulation at defined stages demonstrated that metabolic reprogramming is tightly controlled by the progression through the developmental pathway. The correlation between transcript levels and enzymatic activities in the central metabolism varies significantly in a developmental stage-dependent manner. The complete loss of phosphofructokinase activity at mid-stage meiosis enables a unique setup of the glycolytic pathway which facilitates carbon flux repartitioning into synthesis of spore wall precursors during the co-assimilation of glycogen and acetate. The need for correct homeostasis of purine nucleotides during the meiotic differentiation was demonstrated by the sporulation defect of the AMP deaminase mutant amd1, which exhibited hyper-accumulation of ATP accompanied by depletion of guanosine nucleotides. Conclusions: Our systems-level analysis shows that reprogramming of the central metabolism during the meiotic differentiation is controlled at different hierarchical levels to meet the metabolic and energetic needs at successive developmental stages.</description>
<date>2014</date>
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