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<title lang=en>The Dynamics of Genetic Interactions Between Vibrio metoecus and Vibrio cholerae, Two Close Relatives Co-Occurring in the Environment </title>
<creator>Orata, Fabini</creator>
<creator>Kirchberger, Paul</creator>
<creator>Méheust, Raphaël</creator>
<creator>Barlow, E. Jed</creator>
<creator>Tarr, Cheryl L.</creator>
<creator>Boucher, Yan</creator>
<contributor>University of Alberta [Edmonton]</contributor>
<contributor>Evolution Paris Seine ; Université Nice Sophia Antipolis (UNS) ; Université Côte d'Azur (UCA) - Université Côte d'Azur (UCA) - Centre National de la Recherche Scientifique (CNRS) - Université des Antilles et de la Guyane (UAG) - Université Pierre et Marie Curie - Paris 6 (UPMC)</contributor>
<contributor>Centers for Disease Control and Prevention [Atlanta] (CDC) ; Centers for Disease Control and Prevention</contributor>
<description>International audience</description>
<source>ISSN: 1759-6653</source>
<source>EISSN: 1759-6653</source>
<source>Genome Biology and Evolution</source>
<publisher>Society for Molecular Biology and Evolution</publisher>
<identifier>hal-01264332</identifier>
<identifier>http://hal.upmc.fr/hal-01264332</identifier>
<identifier>http://hal.upmc.fr/hal-01264332/document</identifier>
<identifier>http://hal.upmc.fr/hal-01264332/file/Genome%20Biol%20Evol-2015-Orata-gbe_evv193.pdf</identifier>
<source>http://hal.upmc.fr/hal-01264332</source>
<source>Genome Biology and Evolution, Society for Molecular Biology and Evolution, 2015, 7 (10), pp.2941-2954. 〈10.1093/gbe/evv193〉</source>
<identifier>DOI : 10.1093/gbe/evv193</identifier>
<relation>info:eu-repo/semantics/altIdentifier/doi/10.1093/gbe/evv193</relation>
<language>en</language>
<subject lang=en>Vibrio metoecus</subject>
<subject lang=en>Vibrio cholerae</subject>
<subject lang=en>horizontal gene transfer</subject>
<subject lang=en>genomic islands</subject>
<subject lang=en>integron</subject>
<subject lang=en>comparative genomics</subject>
<subject>[SDV.GEN] Life Sciences [q-bio]/Genetics</subject>
<type>info:eu-repo/semantics/article</type>
<type>Journal articles</type>
<description lang=en>Vibrio metoecus is the closest relative of Vibrio cholerae, the causative agent of the potent diarrheal disease cholera. Although the pathogenic potential of this new species is yet to be studied in depth, it has been co-isolated with V. cholerae in coastal waters and found in clinical specimens in the USA. We used these two organisms to investigate the genetic interaction between closely related species in their natural environment. The genomes of twenty V. cholerae and four V. metoecus strains isolated from a brackish coastal pond on the US east coast, as well as four clinical V. metoecus strains were sequenced and compared to reference strains. Whole genome comparison shows 86-87% average nucleotide identity (ANI) in their core genes between the two species. On the other hand, the chromosomal integron, which occupies approximately 3% of their genomes, shows higher conservation in ANI between species than any other region of their genomes. The ANI of 93-94% observed in this region is not significantly greater within than between species, meaning that it does not follow species boundaries. V. metoecus does not encode toxigenic V. cholerae major virulence factors, the cholera toxin and toxin co-regulated pilus. However, some of the pathogenicity islands found in pandemic V. cholerae were either present in the common ancestor it shares with V. metoecus, or acquired by clinical and environmental V. metoecus in partial fragments. The virulence factors of V. cholerae are therefore both more ancient and more widespread than previously believed. There is high interspecies recombination in the core genome, which has been detected in 24% of the single-copy core genes, including genes involved in pathogenicity. V. metoecus was six times more often the recipient of DNA from V. cholerae as it was the donor, indicating a strong bias in the direction of gene transfer in the environment.</description>
<rights>http://creativecommons.org/licenses/by/</rights>
<date>2015-10-09</date>
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