untitled
<OAI-PMH schemaLocation=http://www.openarchives.org/OAI/2.0/ http://www.openarchives.org/OAI/2.0/OAI-PMH.xsd>
<responseDate>2018-01-17T12:07:07Z</responseDate>
<request identifier=oai:HAL:hal-01546170v1 verb=GetRecord metadataPrefix=oai_dc>http://api.archives-ouvertes.fr/oai/hal/</request>
<GetRecord>
<record>
<header>
<identifier>oai:HAL:hal-01546170v1</identifier>
<datestamp>2018-01-11</datestamp>
<setSpec>type:ART</setSpec>
<setSpec>subject:sdv</setSpec>
<setSpec>collection:EVOLUTION_PARIS_SEINE</setSpec>
<setSpec>collection:UPMC</setSpec>
<setSpec>collection:EVOL_PARIS_SEINE-SM</setSpec>
<setSpec>collection:CNRS</setSpec>
<setSpec>collection:UNIV-AG</setSpec>
<setSpec>collection:UNICE</setSpec>
<setSpec>collection:SAE</setSpec>
<setSpec>collection:GIP-BE</setSpec>
<setSpec>collection:UPMC_POLE_4</setSpec>
<setSpec>collection:IBPS</setSpec>
<setSpec>collection:UCA-TEST</setSpec>
<setSpec>collection:UNIV-COTEDAZUR</setSpec>
</header>
<metadata><dc>
<publisher>HAL CCSD</publisher>
<title lang=en>DsRed-mediated oligomerization stabilizes HMGB1 on chromatin in vivo and on DNA in vitro</title>
<creator>Messmer, Melanie</creator>
<creator>Klein, Christophe</creator>
<creator>Boniface, Rachel</creator>
<creator>Gnaedig, Nina F.</creator>
<creator>Lecerf, Maxime</creator>
<creator>Barnay-Verdier, Stephanie</creator>
<creator>Marechal, Vincent</creator>
<contributor>Symbiose Marine (SM) ; Systématique, adaptation, évolution (SAE) ; Université Pierre et Marie Curie - Paris 6 (UPMC) - Centre National de la Recherche Scientifique (CNRS) - Université Pierre et Marie Curie - Paris 6 (UPMC) - Centre National de la Recherche Scientifique (CNRS) - Evolution Paris Seine ; Université Nice Sophia Antipolis (UNS) ; Université Côte d'Azur (UCA) - Université Côte d'Azur (UCA) - Centre National de la Recherche Scientifique (CNRS) - Université des Antilles et de la Guyane (UAG) - Université Pierre et Marie Curie - Paris 6 (UPMC) - Université Nice Sophia Antipolis (UNS) ; Université Côte d'Azur (UCA) - Université Côte d'Azur (UCA) - Université des Antilles et de la Guyane (UAG)</contributor>
<description>International audience</description>
<source>ISSN: 0300-9084</source>
<source>Biochimie</source>
<publisher>Elsevier</publisher>
<identifier>hal-01546170</identifier>
<identifier>https://hal.archives-ouvertes.fr/hal-01546170</identifier>
<source>https://hal.archives-ouvertes.fr/hal-01546170</source>
<source>Biochimie, Elsevier, 2013, 95 (4), pp.962-966. 〈10.1016/j.biochi.2012.11.001〉</source>
<identifier>DOI : 10.1016/j.biochi.2012.11.001</identifier>
<relation>info:eu-repo/semantics/altIdentifier/doi/10.1016/j.biochi.2012.11.001</relation>
<language>en</language>
<subject lang=en>HMGB1 mobility</subject>
<subject lang=en> DsRed-mediated oligomerization</subject>
<subject lang=en> Chromatin</subject>
<subject lang=en> FRAP</subject>
<subject>[SDV.BID.EVO] Life Sciences [q-bio]/Biodiversity/Populations and Evolution [q-bio.PE]</subject>
<type>info:eu-repo/semantics/article</type>
<type>Journal articles</type>
<description lang=en>High-mobility group box-1 (HMGB1) is remarkably mobile in living cells, which reflects its ability to interact only transiently with both DNA and protein. This property is likely essential for HMGB1 nuclear activities. Nonetheless the weak interaction of HMGB1 with DNA and/or protein partners has also been a major limitation for investigating HMGB1 subnuclear localisation and for the identification of HMGB1 containing complexes by conventional biochemical approaches. In the present study, FRAP experiments demonstrated that DsRed-mediated oligomerization strongly reduces HMGB1 mobility due to an increased affinity for cellular chromatin. Moreover, oligomerized DsRed HMGB1 exhibited a higher affinity for supercoiled DNA in vitro compared to its monomeric counterpart. These results indicate that DsRed-meditated oligomerization is prone to stabilize labile interactions involving HMGB1 both in vivo and in vitro.</description>
<date>2013-04</date>
</dc>
</metadata>
</record>
</GetRecord>
</OAI-PMH>
