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<datestamp>2017-12-21</datestamp>
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<title lang=en>Ghrelin induces clock gene expression in the liver of goldfish in vitro via protein kinase C and protein kinase A pathways</title>
<creator>Sánchez-Bretaño, Aída</creator>
<creator>Blanco, Ayelén M.</creator>
<creator>Alonso-Gomez, Angel L.</creator>
<creator>Delgado, Maria J.</creator>
<creator>Kah, Olivier</creator>
<creator>Isorna, Esther</creator>
<contributor>Universidad Complutense de Madrid [Madrid] (UCM)</contributor>
<contributor>Institut de recherche, santé, environnement et travail [Rennes] (Irset) ; Université d'Angers (UA) - Université des Antilles et de la Guyane (UAG) - Université de Rennes 1 (UR1) - École des Hautes Études en Santé Publique [EHESP] (EHESP) - Institut National de la Santé et de la Recherche Médicale (INSERM) - Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique )</contributor>
<contributor>Ministerio de Economia y Competitividad [project MINECO] [AGL2016-74857-C3-2-R]</contributor>
<contributor> Ministerio de Economia y Competitividad and Ministerio de Educacion</contributor>
<description>International audience</description>
<source>Journal of Experimental Biology</source>
<publisher>Cambridge University Press</publisher>
<identifier>hal-01519254</identifier>
<identifier>https://hal-univ-rennes1.archives-ouvertes.fr/hal-01519254</identifier>
<identifier>https://hal-univ-rennes1.archives-ouvertes.fr/hal-01519254/document</identifier>
<identifier>https://hal-univ-rennes1.archives-ouvertes.fr/hal-01519254/file/Ghrelin%20induces%20clock%20gene%20expression.pdf</identifier>
<source>https://hal-univ-rennes1.archives-ouvertes.fr/hal-01519254</source>
<source>Journal of Experimental Biology, Cambridge University Press, 2017, 220 (7), pp.1295--1306. 〈10.1242/jeb.144253〉</source>
<identifier>DOI : 10.1242/jeb.144253</identifier>
<relation>info:eu-repo/semantics/altIdentifier/doi/10.1242/jeb.144253</relation>
<identifier>PUBMED : 28126833</identifier>
<relation>info:eu-repo/semantics/altIdentifier/pmid/28126833</relation>
<language>en</language>
<subject lang=en> teleost</subject>
<subject lang=en>orexigenic peptides</subject>
<subject lang=en> circadian system</subject>
<subject lang=en> protein kinasec</subject>
<subject lang=en> protein kinase a</subject>
<subject lang=en> hepatic clock</subject>
<subject>[SDV.MHEP.EM] Life Sciences [q-bio]/Human health and pathology/Endocrinology and metabolism</subject>
<subject>[SDV.BBM] Life Sciences [q-bio]/Biochemistry, Molecular Biology</subject>
<subject>[SDV.BA.ZV] Life Sciences [q-bio]/Animal biology/Vertebrate Zoology</subject>
<type>info:eu-repo/semantics/article</type>
<type>Journal articles</type>
<description lang=en>The liver is the most important link between the circadian system and metabolism. As a food-entrainable oscillator, the hepatic clock needs to be entrained by food-related signals. The objective of the present study was to investigate the possible role of ghrelin (an orexigenic peptide mainly synthesized in the gastrointestinal tract) as an endogenous synchronizer of the liver oscillator in teleosts. To achieve this aim, we first examined the presence of ghrelin receptors in the liver of goldfish. Then, the ghrelin regulation of clock gene expression in the goldfish liver was studied. Finally, the possible involvement of the phospholipase C/ protein kinase C (PLC/ PKC) and adenylate cyclase/protein kinase A (AC/PKA) intracellular signalling pathways was investigated. Ghrelin receptor transcripts, ghs-r1a, are present in the majority of goldfish hepatic cells. Ghrelin induced the mRNA expression of the positive (gbmal1a, gclock1a) and negative (gper genes) elements of the main loop of the molecular clock machinery, as well as grev-erba (auxiliary loop) in cultured liver. These effects were blocked, at least in part, by a ghrelin antagonist. Incubation of liver with a PLC inhibitor (U73122), a PKC activator (phorbol 12-myristate 13-acetate) and a PKC inhibitor (chelerythrine chloride) demonstrated that the PLC/ PKC pathway mediates such ghrelin actions. Experiments with an AC activator (forskolin) and a PKA inhibitor (H89) showed that grev-erba regulation could be due to activation of PKA. Taken together, the present results show for the first time in vertebrates a direct action of ghrelin on hepatic clock genes and support a role for this hormone as a temporal messenger in the entrainment of liver circadian functions.</description>
<date>2017</date>
</dc>
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