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<title lang=en>Global human tissue profiling and protein network analysis reveals distinct levels of transcriptional germline-specificity and identifies target genes for male infertility.</title>
<creator>Chalmel, Frédéric</creator>
<creator>Lardenois, Aurélie</creator>
<creator>Evrard, Bertrand</creator>
<creator>Mathieu, Romain</creator>
<creator>Feig, Caroline</creator>
<creator>Demougin, Philippe</creator>
<creator>Gattiker, Alexandre</creator>
<creator>Schulze, Wolfgang</creator>
<creator>Jégou, Bernard</creator>
<creator>Kirchhoff, Christiane</creator>
<creator>Primig, Michael</creator>
<contributor>Transcriptional networks in gametogenesis and cancer ; Institut de recherche, santé, environnement et travail [Rennes] (Irset) ; Université d'Angers (UA) - Université des Antilles et de la Guyane (UAG) - Université de Rennes 1 (UR1) - École des Hautes Études en Santé Publique [EHESP] (EHESP) - Institut National de la Santé et de la Recherche Médicale (INSERM) - Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ) - Université d'Angers (UA) - Université des Antilles et de la Guyane (UAG) - Université de Rennes 1 (UR1) - École des Hautes Études en Santé Publique [EHESP] (EHESP) - Institut National de la Santé et de la Recherche Médicale (INSERM) - Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique )</contributor>
<contributor>Institut de recherche, santé, environnement et travail [Rennes] (Irset) ; Université d'Angers (UA) - Université des Antilles et de la Guyane (UAG) - Université de Rennes 1 (UR1) - École des Hautes Études en Santé Publique [EHESP] (EHESP) - Institut National de la Santé et de la Recherche Médicale (INSERM) - Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique )</contributor>
<contributor>École des Hautes Études en Santé Publique [EHESP] (EHESP)</contributor>
<contributor>Department of Andrology ; Universitaetsklinikum Hamburg-Eppendorf = University Medical Center Hamburg-Eppendorf [Hamburg] (UKE)</contributor>
<contributor>Biozentrum ; University of Basel (Unibas)</contributor>
<contributor>School of Life Sciences [Lausanne] ; Ecole Polytechnique Fédérale de Lausanne (EPFL)</contributor>
<contributor>Environnement viral et chimique & reproduction ; Institut de recherche, santé, environnement et travail [Rennes] (Irset) ; Université d'Angers (UA) - Université des Antilles et de la Guyane (UAG) - Université de Rennes 1 (UR1) - École des Hautes Études en Santé Publique [EHESP] (EHESP) - Institut National de la Santé et de la Recherche Médicale (INSERM) - Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ) - Université d'Angers (UA) - Université des Antilles et de la Guyane (UAG) - Université de Rennes 1 (UR1) - École des Hautes Études en Santé Publique [EHESP] (EHESP) - Institut National de la Santé et de la Recherche Médicale (INSERM) - Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique )</contributor>
<description>International audience</description>
<source>ISSN: 0268-1161</source>
<source>EISSN: 1460-2350</source>
<source>Human Reproduction</source>
<publisher>Oxford University Press (OUP)</publisher>
<identifier>hal-00877243</identifier>
<identifier>https://hal.archives-ouvertes.fr/hal-00877243</identifier>
<source>https://hal.archives-ouvertes.fr/hal-00877243</source>
<source>Human Reproduction, Oxford University Press (OUP), 2012, 27 (11), pp.3233-48. 〈10.1093/humrep/des301〉</source>
<identifier>DOI : 10.1093/humrep/des301</identifier>
<relation>info:eu-repo/semantics/altIdentifier/doi/10.1093/humrep/des301</relation>
<identifier>PUBMED : 22926843</identifier>
<relation>info:eu-repo/semantics/altIdentifier/pmid/22926843</relation>
<language>en</language>
<subject lang=en>Male genital diseases</subject>
<subject lang=en>Vertebrata</subject>
<subject lang=en>Human</subject>
<subject lang=en>Male sterility</subject>
<subject lang=en>Mammalia</subject>
<subject lang=en>Spermatogenesis</subject>
<subject lang=en>Gene</subject>
<subject lang=en>Target</subject>
<subject lang=en>Specificity</subject>
<subject lang=en>Network</subject>
<subject lang=en>Protein</subject>
<subject lang=en>Tissue</subject>
<subject>[SDV.BDLR] Life Sciences [q-bio]/Reproductive Biology</subject>
<type>info:eu-repo/semantics/article</type>
<type>Journal articles</type>
<description lang=en>BACKGROUND: Mammalian spermatogenesis is a process that involves a complex expression program in both somatic and germ cells present in the male gonad. A number of studies have attempted to define the transcriptome of male meiosis and gametogenesis in rodents and primates. Few human transcripts, however, have been associated with testicular somatic cells and germ cells at different post-natal developmental stages and little is known about their level of germline-specificity compared with non-testicular tissues. METHODS: We quantified human transcripts using GeneChips and a total of 47 biopsies from prepubertal children diagnosed with undescended testis, infertile adult patients whose spermatogenesis is arrested at consecutive stages and fertile control individuals. These results were integrated with data from enriched normal germ cells, non-testicular expression data, phenotype information, predicted regulatory DNA-binding motifs and interactome data. RESULTS: Among 3580 genes for which we found differential transcript concentrations in somatic and germ cells present in human testis, 933 were undetectable in 45 embryonic and adult non-testicular tissues, including many that were corroborated at protein level by published gene annotation data and histological high-throughput protein immunodetection assays. Using motif enrichment analyses, we identified regulatory promoter elements likely involved in germline development. Finally, we constructed a regulatory disease network for human fertility by integrating expression signals, interactome information, phenotypes and functional annotation data. CONCLUSIONS: Our results provide broad insight into the post-natal human testicular transcriptome at the level of cell populations and in a global somatic tissular context. Furthermore, they yield clues for genetic causes of male infertility and will facilitate the identification of novel cancer/testis genes as targets for cancer immunotherapies.</description>
<date>2012-11</date>
</dc>
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