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<datestamp>2018-01-12</datestamp>
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<publisher>HAL CCSD</publisher>
<title lang=en>Genomics and genetics of Sulfolobus islandicus LAL14/1, a model hyperthermophilic archaeon.</title>
<creator>Jaubert, Carole</creator>
<creator>Danioux, Chloë</creator>
<creator>Oberto, Jacques</creator>
<creator>Cortez, Diego</creator>
<creator>Bize, Ariane</creator>
<creator>Krupovic, Mart</creator>
<creator>She, Qunxin</creator>
<creator>Forterre, Patrick</creator>
<creator>Prangishvili, David</creator>
<creator>Sezonov, Guennadi</creator>
<contributor>Institut de génétique et microbiologie [Orsay] (IGM) ; Université Paris-Sud - Paris 11 (UP11) - Centre National de la Recherche Scientifique (CNRS)</contributor>
<contributor>Biologie Moléculaire du Gène chez les Extrêmophiles (BMGE) ; Institut Pasteur [Paris]</contributor>
<contributor>Génomique et Génétique des thaumarchées (GGT) ; Systématique, adaptation, évolution (SAE) ; Université Pierre et Marie Curie - Paris 6 (UPMC) - Centre National de la Recherche Scientifique (CNRS) - Université Pierre et Marie Curie - Paris 6 (UPMC) - Centre National de la Recherche Scientifique (CNRS) - Evolution Paris Seine ; Université Nice Sophia Antipolis (UNS) ; Université Côte d'Azur (UCA) - Université Côte d'Azur (UCA) - Centre National de la Recherche Scientifique (CNRS) - Université des Antilles et de la Guyane (UAG) - Université Pierre et Marie Curie - Paris 6 (UPMC) - Université Nice Sophia Antipolis (UNS) ; Université Côte d'Azur (UCA) - Université Côte d'Azur (UCA) - Université des Antilles et de la Guyane (UAG)</contributor>
<description>International audience</description>
<source>ISSN: 2046-6390</source>
<source>Biology Open</source>
<publisher>Royal Society</publisher>
<identifier>hal-00818894</identifier>
<identifier>https://hal.archives-ouvertes.fr/hal-00818894</identifier>
<source>https://hal.archives-ouvertes.fr/hal-00818894</source>
<source>Biology Open, Royal Society, 2013, 3 (4), pp.130010. 〈10.1098/rsob.130010〉</source>
<identifier>DOI : 10.1098/rsob.130010</identifier>
<relation>info:eu-repo/semantics/altIdentifier/doi/10.1098/rsob.130010</relation>
<identifier>PUBMED : 23594878</identifier>
<relation>info:eu-repo/semantics/altIdentifier/pmid/23594878</relation>
<language>en</language>
<subject>[SDV.BBM] Life Sciences [q-bio]/Biochemistry, Molecular Biology</subject>
<subject>[SDV.MP] Life Sciences [q-bio]/Microbiology and Parasitology</subject>
<type>info:eu-repo/semantics/article</type>
<type>Journal articles</type>
<description lang=en>The 2 465 177 bp genome of Sulfolobus islandicus LAL14/1, host of the model rudivirus SIRV2, was sequenced. Exhaustive comparative genomic analysis of S. islandicus LAL14/1 and the nine other completely sequenced S. islandicus strains isolated from Iceland, Russia and USA revealed a highly syntenic common core genome of approximately 2 Mb and a long hyperplastic region containing most of the strain-specific genes. In LAL14/1, the latter region is enriched in insertion sequences, CRISPR (clustered regularly interspaced short palindromic repeats), glycosyl transferase genes, toxin-antitoxin genes and MITE (miniature inverted-repeat transposable elements). The tRNA genes of LAL14/1 are preferential targets for the integration of mobile elements but clusters of atypical genes (CAG) are also integrated elsewhere in the genome. LAL14/1 carries five CRISPR loci with 10 per cent of spacers matching perfectly or imperfectly the genomes of archaeal viruses and plasmids found in the Icelandic hot springs. Strikingly, the CRISPR_2 region of LAL14/1 carries an unusually long 1.9 kb spacer interspersed between two repeat regions and displays a high similarity to pING1-like conjugative plasmids. Finally, we have developed a genetic system for S. islandicus LAL14/1 and created ΔpyrEF and ΔCRISPR_1 mutants using double cross-over and pop-in/pop-out approaches, respectively. Thus, LAL14/1 is a promising model to study virus-host interactions and the CRISPR/Cas defence mechanism in Archaea.</description>
<date>2013</date>
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