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<publisher>HAL CCSD</publisher>
<title lang=en>Immunoreactive proteins of Saccharopolyspora rectivirgula for farmer's lung serodiagnosis.</title>
<creator>Barrera, Coralie</creator>
<creator>Millon, Laurence</creator>
<creator>Rognon, Bénédicte</creator>
<creator>Quadroni, Manfredo</creator>
<creator>Roussel, Sandrine</creator>
<creator>Dalphin, Jean-Charles</creator>
<creator>Court-Fortune, Isabelle</creator>
<creator>Caillaud, Denis</creator>
<creator>Jouneau, Stephane</creator>
<creator>Fellrath, Jean-Marc</creator>
<creator>Zaugg, Christophe</creator>
<creator>Reboux, Gabriel</creator>
<creator>Monod, Michel</creator>
<contributor>Laboratoire Chrono-environnement (LCE) ; Université Bourgogne Franche-Comté (UBFC) - Centre National de la Recherche Scientifique (CNRS) - Université de Franche-Comté (UFC)</contributor>
<contributor>Centre Intégratif de Génomique, plateforme de protéomique, ; Centre Intégratif de Génomique,</contributor>
<contributor>Service de Pneumologie [Saint-Etienne] ; CHU Saint-Etienne</contributor>
<contributor>Service de Pneumologie et Allergologie ; CHU Clermont-Ferrand</contributor>
<contributor>Institut de recherche, santé, environnement et travail [Rennes] (Irset) ; Université d'Angers (UA) - Université des Antilles et de la Guyane (UAG) - Université de Rennes 1 (UR1) - École des Hautes Études en Santé Publique [EHESP] (EHESP) - Institut National de la Santé et de la Recherche Médicale (INSERM) - Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique )</contributor>
<contributor>Technologies et systèmes d'information pour les agrosystèmes (UR TSCF) ; Institut national de recherche en sciences et technologies pour l'environnement et l'agriculture (IRSTEA)</contributor>
<description>International audience</description>
<source>ISSN: 1862-8346</source>
<source>EISSN: 1862-8354</source>
<source>PROTEOMICS - Clinical Applications</source>
<publisher>Wiley-VCH Verlag</publisher>
<identifier>hal-01115880</identifier>
<identifier>https://hal.archives-ouvertes.fr/hal-01115880</identifier>
<source>https://hal.archives-ouvertes.fr/hal-01115880</source>
<source>PROTEOMICS - Clinical Applications, Wiley-VCH Verlag, 2014, 8 (11-12), pp.971-81. 〈10.1002/prca.201400024〉</source>
<identifier>DOI : 10.1002/prca.201400024</identifier>
<relation>info:eu-repo/semantics/altIdentifier/doi/10.1002/prca.201400024</relation>
<identifier>PUBMED : 25264606</identifier>
<relation>info:eu-repo/semantics/altIdentifier/pmid/25264606</relation>
<language>en</language>
<subject>[SDV.EE.SANT] Life Sciences [q-bio]/Ecology, environment/Health</subject>
<type>info:eu-repo/semantics/article</type>
<type>Journal articles</type>
<description lang=en>Saccharopolyspora rectivirgula is the principal cause of farmer's lung disease (FLD). Serodiagnosis is based on immunoprecipitation techniques or enzyme immunoassays with homemade crude antigens and is not standardized. We aimed to produce specific recombinant antigens for the development of a standardized ELISA. We recruited 41 patients and 43 healthy exposed controls from five university hospital pneumology departments in France and Switzerland. S. rectivirgula proteins were extracted, separated by 2D electrophoresis, and subjected to Western blotting, with sera from FLD patients or controls. FLD-specific proteins were identified by MS and were produced as recombinant antigens. The diagnostic performance of ELISA tests using the recombinant antigens was assessed with all the sera from FLD patients and controls. We identified 25 FLD-specific proteins, some of which play important roles in transport, nutrition, or virulence. We produced 17 of these proteins as recombinant antigens and assessed their suitability for inclusion in the ELISA test. A combination of three of these proteins (SR1FA, SR17, and SR22) proved remarkably effective at discriminating between patients and controls, with a sensitivity of 83% and a specificity of 77%. The recombinant antigens produced in this study constitute a major step toward the improvement of diagnostic performance and the standardization of FLD serodiagnosis.</description>
<date>2014-11-30</date>
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