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<title lang=en>Identification and frequency of circulating CD4(+) T lymphocytes specific to Benzylpenicillin in healthy donors.</title>
<creator>Nhim, C</creator>
<creator>Delluc, S</creator>
<creator>Halgand, F</creator>
<creator>de Chaisemartin, L</creator>
<creator>Weaver, R J</creator>
<creator>Claude, N</creator>
<creator>Joseph, D</creator>
<creator>Maillère, B</creator>
<creator>Pallardy, M</creator>
<contributor>Cytokines, chimiokines et immunopathologie ; Université Paris-Sud - Paris 11 (UP11) - Institut National de la Santé et de la Recherche Médicale (INSERM)</contributor>
<contributor>Institut de recherche, santé, environnement et travail [Rennes] (Irset) ; Université d'Angers (UA) - Université des Antilles et de la Guyane (UAG) - Université de Rennes 1 (UR1) - École des Hautes Études en Santé Publique [EHESP] (EHESP) - Institut National de la Santé et de la Recherche Médicale (INSERM) - Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique )</contributor>
<contributor>Service d'Ingénierie Moléculaire des Protéines (SIMOPRO) ; Commissariat à l'énergie atomique et aux énergies alternatives (CEA) - Université Paris-Saclay</contributor>
<description>International audience</description>
<source>ISSN: 0105-4538</source>
<source>EISSN: 1398-9995</source>
<source>Allergy</source>
<publisher>Wiley</publisher>
<identifier>hal-01122172</identifier>
<identifier>https://hal-univ-rennes1.archives-ouvertes.fr/hal-01122172</identifier>
<source>https://hal-univ-rennes1.archives-ouvertes.fr/hal-01122172</source>
<source>Allergy, Wiley, 2013, 68 (7), pp.899-905. 〈10.1111/all.12173〉</source>
<identifier>PUBMED : 23751122</identifier>
<relation>info:eu-repo/semantics/altIdentifier/pmid/23751122</relation>
<identifier>DOI : 10.1111/all.12173</identifier>
<relation>info:eu-repo/semantics/altIdentifier/doi/10.1111/all.12173</relation>
<language>en</language>
<subject>[SDV] Life Sciences [q-bio]</subject>
<type>info:eu-repo/semantics/article</type>
<type>Journal articles</type>
<description lang=en>Drug hypersensitivity is known to rely on a drug-specific T-cell response. Amplitude of antigen-specific T-cell response is partly controlled by the size of the antigen-specific naïve CD4(+) T-cell repertoire, but estimate of this repertoire has never been investigated for allergenic drugs. The purpose of this study was to evaluate the frequency of benzylpenicillin-specific CD4(+) T lymphocytes in healthy donors. Co-cultures were established with CD4(+) T lymphocytes from healthy donors and mature autologous dendritic cells loaded with benzylpenicillin coupled to human serum albumin. CD4(+) T lymphocytes were stimulated once a week for 4 weeks with benzylpenicillin coupled to human serum albumin. The CD4(+) T-cell response was measured using an interferon-γ ELISPOT assay. Frequency of benzylpenicillin-specific naive CD4(+) T lymphocytes was then calculated using the Poisson distribution law. Results showed the presence of benzylpenicillin-specific CD4(+) T lymphocytes in 9 of 10 tested healthy donors irrespective of their HLA typing, with a mean frequency of 0.29 cells per million of CD4(+) T cells. Experiments performed on naive (CD45RA(+) ) and on memory (CD45RO(+) ) CD4(+) T lymphocytes showed that these benzylpenicillin-specific CD4(+) T lymphocytes belonged to the naive T-cell subpopulation. This study showed for the first time the existence of naive CD4(+) T lymphocytes specific to benzylpenicillin in healthy donors.</description>
<date>2013-06-30</date>
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