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<datestamp>2018-01-11</datestamp>
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<title lang=en>Isolation and characterization of eight microsatellite loci from Galeocerdo cuvier (tiger shark) and cross-amplification in Carcharhinus leucas, Carcharhinus brevipinna, Carcharhinus plumbeus and Sphyrna lewini</title>
<creator>Pirog, Agathe</creator>
<creator>Jaquemet, Sébastien</creator>
<creator>Blaison, Antonin</creator>
<creator>Soria, Marc</creator>
<creator>Magalon, Hélène</creator>
<contributor>Ecologie marine tropicale dans les Océans Pacifique et Indien (ENTROPIE [Réunion]) ; Institut de Recherche pour le Développement (IRD) - Université de la Réunion (UR) - Centre National de la Recherche Scientifique (CNRS)</contributor>
<contributor>UMR 212 EME "écosystèmes marins exploités" (EME) ; Institut de Recherche pour le Développement (IRD) - Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER) - Université de Montpellier (UM)</contributor>
<contributor>Laboratoire d'Excellence CORAIL (LabEX CORAIL) ; Institut de Recherche pour le Développement (IRD) - Université des Antilles et de la Guyane (UAG) - École des hautes études en sciences sociales (EHESS) - École pratique des hautes études (EPHE) - Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER) - Université de la Réunion (UR) - Université de la Polynésie Française (UPF) - Université de Nouvelle Calédonie - Institut d'écologie et environnement</contributor>
<description>International audience</description>
<source>ISSN: 2167-8359</source>
<source>PeerJ</source>
<publisher>PeerJ</publisher>
<identifier>hal-01317961</identifier>
<identifier>https://hal.archives-ouvertes.fr/hal-01317961</identifier>
<identifier>https://hal.archives-ouvertes.fr/hal-01317961/document</identifier>
<identifier>https://hal.archives-ouvertes.fr/hal-01317961/file/Pirog_2016PeerJ_microsat_tiger_shark.pdf</identifier>
<source>https://hal.archives-ouvertes.fr/hal-01317961</source>
<source>PeerJ, PeerJ, 2016, pp.e2041. 〈10.7717/peerj.2041〉</source>
<identifier>DOI : 10.7717/peerj.2041</identifier>
<relation>info:eu-repo/semantics/altIdentifier/doi/10.7717/peerj.2041</relation>
<language>en</language>
<subject lang=en>Carcharhiniform</subject>
<subject lang=en>Microsatellites</subject>
<subject lang=en>Control region</subject>
<subject lang=en>Population Genetics</subject>
<subject>[SDE.BE] Environmental Sciences/Biodiversity and Ecology</subject>
<subject>[SDV.GEN.GPO] Life Sciences [q-bio]/Genetics/Populations and Evolution [q-bio.PE]</subject>
<type>info:eu-repo/semantics/article</type>
<type>Journal articles</type>
<description lang=en>The tiger shark Galeocerdo cuvier (Carcharhinidae) is a large elasmobranch suspected to have, as other apex predators, a keystone function in marine ecosystems and is currently considered Near Threatened (Red list IUCN). Knowledge on its ecology, which is crucial to design proper conservation and management plans, is very scarce. Here we describe the isolation of eight polymorphic microsatellite loci using 454 GS-FLX Titanium pyrosequencing of enriched DNA libraries. Their characteristics were tested on a population of tiger shark (n = 101) from Reunion Island (SouthWestern Indian Ocean). All loci were polymorphic with a number of alleles ranging from two to eight. No null alleles were detected and no linkage disequilibrium was detected after Bonferroni correction. Observed and expected heterozygosities ranged from 0.03 to 0.76 and from 0.03 to 0.77, respectively. No locus deviated from Hardy-Weinberg equilibrium and the global F IS of the population was of 0.04 NS. Some of the eight loci developed here successfully cross-amplified in the bull shark Carcharhinus leucas (one locus), the spinner shark Carcharhinus brevipinna (four loci), the sandbar shark Carcharhinus plumbeus (five loci) and the scalloped hammerhead shark Sphyrna lewini (two loci). We also designed primers to amplify and sequence a mitochondrial marker, the control region. We sequenced 862 bp and found a low genetic diversity, with four polymorphic sites, a haplotype diversity of 0.15 and a nucleotide diversity of 2 × 10 −4 .</description>
<date>2016</date>
</dc>
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