untitled
<OAI-PMH schemaLocation=http://www.openarchives.org/OAI/2.0/ http://www.openarchives.org/OAI/2.0/OAI-PMH.xsd>
<responseDate>2018-01-15T18:28:18Z</responseDate>
<request identifier=oai:HAL:hal-01187142v1 verb=GetRecord metadataPrefix=oai_dc>http://api.archives-ouvertes.fr/oai/hal/</request>
<GetRecord>
<record>
<header>
<identifier>oai:HAL:hal-01187142v1</identifier>
<datestamp>2018-01-11</datestamp>
<setSpec>type:ART</setSpec>
<setSpec>subject:sdv</setSpec>
<setSpec>collection:UNIV-RENNES1</setSpec>
<setSpec>collection:CNRS</setSpec>
<setSpec>collection:UNIV-AG</setSpec>
<setSpec>collection:UNIV-ORLEANS</setSpec>
<setSpec>collection:IRSET</setSpec>
<setSpec>collection:HL</setSpec>
<setSpec>collection:IRSET-HIAEC</setSpec>
<setSpec>collection:IFR140</setSpec>
<setSpec>collection:BIOSIT</setSpec>
<setSpec>collection:UR1-UFR-SVE</setSpec>
<setSpec>collection:STATS-UR1</setSpec>
<setSpec>collection:UR1-SDV</setSpec>
<setSpec>collection:CEA</setSpec>
<setSpec>collection:UR1-HAL</setSpec>
<setSpec>collection:EHESP</setSpec>
<setSpec>collection:USPC</setSpec>
<setSpec>collection:IRSET-2</setSpec>
<setSpec>collection:UNIV-ANGERS</setSpec>
</header>
<metadata><dc>
<publisher>HAL CCSD</publisher>
<title lang=en>Identification, biochemical characterization, and in-vivo expression of the intracellular invertase BfrA from the pathogenic parasite Leishmania major</title>
<creator>Belaz, Sorya</creator>
<creator>Rattier, Thibault</creator>
<creator>Lafite, Pierre</creator>
<creator>Moreau, Philippe</creator>
<creator>Routier, Françoise H.</creator>
<creator>Robert-Gangneux, Florence</creator>
<creator>Gangneux, Jean-Pierre</creator>
<creator>Daniellou, Richard</creator>
<contributor>Institut de recherche, santé, environnement et travail [Rennes] (Irset) ; Université d'Angers (UA) - Université des Antilles et de la Guyane (UAG) - Université de Rennes 1 (UR1) - École des Hautes Études en Santé Publique [EHESP] (EHESP) - Institut National de la Santé et de la Recherche Médicale (INSERM) - Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique )</contributor>
<contributor>Service de Parasitologie-Mycologie [Rennes] ; Université de Rennes 1 (UR1) - Hôpital Pontchaillou - CHU Pontchaillou [Rennes]</contributor>
<contributor>Institut de Chimie Organique et Analytique (ICOA) ; Université d'Orléans (UO) - Centre National de la Recherche Scientifique (CNRS) - Commissariat à l'énergie atomique et aux énergies alternatives (CEA)</contributor>
<description>International audience</description>
<source>ISSN: 0008-6215</source>
<source>EISSN: 0008-6215</source>
<source>Carbohydrate Research</source>
<publisher>Elsevier</publisher>
<identifier>hal-01187142</identifier>
<identifier>https://hal-univ-rennes1.archives-ouvertes.fr/hal-01187142</identifier>
<source>https://hal-univ-rennes1.archives-ouvertes.fr/hal-01187142</source>
<source>Carbohydrate Research, Elsevier, 2015, 415, pp.31--38. 〈10.1016/j.carres.2015.07.001〉</source>
<identifier>DOI : 10.1016/j.carres.2015.07.001</identifier>
<relation>info:eu-repo/semantics/altIdentifier/doi/10.1016/j.carres.2015.07.001</relation>
<identifier>PUBMED : 26279524</identifier>
<relation>info:eu-repo/semantics/altIdentifier/pmid/26279524</relation>
<language>en</language>
<subject lang=en>Leishmania</subject>
<subject lang=en>Leishmaniasis</subject>
<subject lang=en>Sucrose</subject>
<subject lang=en>Invertase</subject>
<subject lang=en>Glycosylation</subject>
<subject>[SDV] Life Sciences [q-bio]</subject>
<type>info:eu-repo/semantics/article</type>
<type>Journal articles</type>
<description lang=en>The parasitic life cycle of Leishmania includes an extracellular promastigote stage that occurs in the gut of the insect vector. During that period, the sucrose metabolism and more specifically the first glycosidase of this pathway are essential for growth and survival of the parasite. We investigated the expression of the invertase BfrA in the promastigote and amastigote stages of three parasite species representative of the three various clinical forms and of various geographical areas, namely Leishmania major, L. donovani and L. braziliensis. Thereafter, we cloned, overexpressed and biochemically characterized this invertase BfrA from L. major, heterologously expressed in both Escherichia coli and L. tarentolae. For all species, expression levels of BfrA mRNA were correlated to the time of the culture and the parasitic stage (promastigotes > amastigotes). BfrA exhibited no activity when expressed as a glycoprotein in L. tarentolae but proved to be an invertase when not glycosylated, yet owing low sequence homology with other invertases from the same family. Our data suggest that BfrA is an original invertase that is located inside the parasite. It is expressed in both parasitic stages, though to a higher extent in promastigotes. This work provides new insight into the parasite sucrose metabolism</description>
<date>2015</date>
</dc>
</metadata>
</record>
</GetRecord>
</OAI-PMH>
