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<title lang=en>Identification of a Marine Cyanophage in a Protist Single-cell Metagenome Assembly</title>
<creator>Bhattacharya, Debashish</creator>
<creator>Price, Dana C.</creator>
<creator>Bicep, Cedric</creator>
<creator>Bapteste, Eric</creator>
<creator>Sarwade, Mihir</creator>
<creator>Rajah, Veeran D.</creator>
<creator>Yoon, Hwan Su</creator>
<contributor>Adaptation, Intégration, Réticulation et Evolution (AIRE) ; Systématique, adaptation, évolution (SAE) ; Université Pierre et Marie Curie - Paris 6 (UPMC) - Centre National de la Recherche Scientifique (CNRS) - Université Pierre et Marie Curie - Paris 6 (UPMC) - Centre National de la Recherche Scientifique (CNRS) - Evolution Paris Seine ; Université Nice Sophia Antipolis (UNS) ; Université Côte d'Azur (UCA) - Université Côte d'Azur (UCA) - Centre National de la Recherche Scientifique (CNRS) - Université des Antilles et de la Guyane (UAG) - Université Pierre et Marie Curie - Paris 6 (UPMC) - Université Nice Sophia Antipolis (UNS) ; Université Côte d'Azur (UCA) - Université Côte d'Azur (UCA) - Université des Antilles et de la Guyane (UAG)</contributor>
<contributor>NSF [EF-0827023, DEB-0936884, DEB-0937975]</contributor>
<contributor> Next-Generation BioGreen 21 Program (SSAC), Rural Development Administration in Korea [2012-PJ008177]</contributor>
<description>International audience</description>
<source>ISSN: 0022-3646</source>
<source>EISSN: 1529-8817</source>
<source>Journal of Phycology</source>
<publisher>Wiley</publisher>
<identifier>hal-01544797</identifier>
<identifier>https://hal.archives-ouvertes.fr/hal-01544797</identifier>
<source>https://hal.archives-ouvertes.fr/hal-01544797</source>
<source>Journal of Phycology, Wiley, 2013, 49 (1), pp.207-212. 〈10.1111/jpy.12028〉</source>
<identifier>DOI : 10.1111/jpy.12028</identifier>
<relation>info:eu-repo/semantics/altIdentifier/doi/10.1111/jpy.12028</relation>
<language>en</language>
<subject lang=en>cyanophage</subject>
<subject lang=en> gene network</subject>
<subject lang=en> horizontal gene transfer</subject>
<subject lang=en> Paulinella</subject>
<subject lang=en> phylogenomics</subject>
<subject lang=en> single cell genomics</subject>
<subject>[SDV.BID] Life Sciences [q-bio]/Biodiversity</subject>
<type>info:eu-repo/semantics/article</type>
<type>Journal articles</type>
<description lang=en>Analysis of microbial biodiversity is hampered by a lack of reference genomes from most bacteria, viruses, and algae. This necessitates either the cultivation of a restricted number of species for standard sequencing projects or the analysis of highly complex environmental DNA metagenome data. Single-cell genomics (SCG) offers a solution to this problem by constraining the studied DNA sample to an individual cell and its associated symbionts, prey, and pathogens. We used SCG to study marine heterotrophic amoebae related to Paulinella ovalis (A. Wulff) P.W. Johnson, P.E. Hargraves & J.M. Sieburth (Rhizaria). The genus Paulinella is best known for its photosynthetic members such as P.chromatophora Lauterborn that is the only case of plastid primary endosymbiosis known outside of algae and plants. Here, we studied the phagotrophic sister taxa of P.chromatophora that are related to P.ovalis and found one SCG assembly to contain -cyanobacterial DNA. These cyanobacterial contigs are presumably derived from prey. We also uncovered an associated cyanophage lineage (provisionally named phage PoL_MC2). Phylogenomic analysis of the fragmented genome assembly suggested a minimum genome size of 200Kbp for phage PoL_MC2 that encodes 179 proteins and is most closely related to Synechococcus phage S-SM2. For this phage, gene network analysis demonstrates a highly modular genome structure typical of other cyanophages. Our work demonstrates that SCG is a powerful approach for discovering algal and protist biodiversity and for elucidating biotic interactions in natural samples.</description>
<date>2013-02</date>
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