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<datestamp>2018-01-12</datestamp>
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<publisher>HAL CCSD</publisher>
<title lang=en>In Silico Analyses of Primers Used to Detect the Pathogenicity Genes of Vibrio cholerae</title>
<creator>Gardes, Julien</creator>
<creator>Croce, Olivier</creator>
<creator>Christen, Richard</creator>
<contributor>Symbiose Marine (SM) ; Systématique, adaptation, évolution (SAE) ; Université Pierre et Marie Curie - Paris 6 (UPMC) - Centre National de la Recherche Scientifique (CNRS) - Université Pierre et Marie Curie - Paris 6 (UPMC) - Centre National de la Recherche Scientifique (CNRS) - Evolution Paris Seine ; Université Nice Sophia Antipolis (UNS) ; Université Côte d'Azur (UCA) - Université Côte d'Azur (UCA) - Centre National de la Recherche Scientifique (CNRS) - Université des Antilles et de la Guyane (UAG) - Université Pierre et Marie Curie - Paris 6 (UPMC) - Université Nice Sophia Antipolis (UNS) ; Université Côte d'Azur (UCA) - Université Côte d'Azur (UCA) - Université des Antilles et de la Guyane (UAG)</contributor>
<contributor>Delegation Generale pour l'Armement (DGA), Ministere de la Defense Francaise</contributor>
<contributor> PICS</contributor>
<description>International audience</description>
<source>ISSN: 1342-6311</source>
<source>EISSN: 1347-4405</source>
<source>Microbes and environments / JSME</source>
<publisher>Nakanishi Printing Co</publisher>
<identifier>hal-01546178</identifier>
<identifier>https://hal.archives-ouvertes.fr/hal-01546178</identifier>
<source>https://hal.archives-ouvertes.fr/hal-01546178</source>
<source>Microbes and environments / JSME, Nakanishi Printing Co, 2012, 27 (3), pp.250-256. 〈10.1264/jsme2.ME11317〉</source>
<identifier>DOI : 10.1264/jsme2.ME11317</identifier>
<relation>info:eu-repo/semantics/altIdentifier/doi/10.1264/jsme2.ME11317</relation>
<language>en</language>
<subject lang=en>primers</subject>
<subject lang=en> Vibrio cholerae</subject>
<subject lang=en> virulence genes</subject>
<subject>[SDV.BID.EVO] Life Sciences [q-bio]/Biodiversity/Populations and Evolution [q-bio.PE]</subject>
<type>info:eu-repo/semantics/article</type>
<type>Journal articles</type>
<description lang=en>In Vibrio cholerae, the etiological agent of cholera, most of the virulence genes are located in two pathogenicity islands, named TCP (Toxin-Co-regulated Pilus) and CTX (Cholera ToXins). For each V. cholerae pathogenicity gene, we retrieved every primer published since 1990 and every known allele in order to perform a complete in silico survey and assess the quality of the PCR primers used for amplification of these genes. Primers with a melting temperature in the range 55-60 degrees C against any target sequence were considered valid. Our survey clearly revealed that two thirds of the published primers are not able to properly detect every genetic variant of the target genes. Moreover, the quality of primers did not improve with time. Their lifetime, i.e. the number of times they were cited in the literature, is also not a factor allowing the selection of valid primers. We were able to improve some primers or design new primers for the few cases where no valid primer was found. In conclusion, many published primers should be avoided or improved for use in molecular detection tests, in order to improve and perfect specificity and coverage. This study suggests that bioinformatic analyses are important to validate the choice of primers.</description>
<date>2012-09</date>
</dc>
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