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<datestamp>2018-01-11</datestamp>
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<publisher>HAL CCSD</publisher>
<title lang=en>Identification of antigenic proteins from lichtheimia corymbifera for farmer's lung disease diagnosis</title>
<creator>Rognon, Bénédicte</creator>
<creator>Barrera, C.</creator>
<creator>Monod, M.</creator>
<creator>Valot, B.</creator>
<creator>Roussel, S.</creator>
<creator>Quadroni, M.</creator>
<creator>Jouneau, S.</creator>
<creator>Court-Fortune, I.</creator>
<creator>Caillaud, D.</creator>
<creator>Fellrath, J.-M.</creator>
<creator>Dalphin, J.-C.</creator>
<creator>Reboux, G.</creator>
<creator>Millon, L.</creator>
<contributor>Laboratoire Chrono-environnement (LCE) ; Université Bourgogne Franche-Comté (UBFC) - Université de Franche-Comté (UFC) - Centre National de la Recherche Scientifique (CNRS)</contributor>
<contributor>Institut de biologie et chimie des protéines [Lyon] (IBCP) ; Université Claude Bernard Lyon 1 (UCBL) - Centre National de la Recherche Scientifique (CNRS)</contributor>
<contributor>Institut de recherche, santé, environnement et travail [Rennes] (Irset) ; Université d'Angers (UA) - Université des Antilles et de la Guyane (UAG) - Université de Rennes 1 (UR1) - École des Hautes Études en Santé Publique [EHESP] (EHESP) - Institut National de la Santé et de la Recherche Médicale (INSERM) - Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique )</contributor>
<contributor>CHU Pontchaillou [Rennes]</contributor>
<contributor>Service de Pneumologie [Saint-Etienne] ; CHU Saint-Etienne</contributor>
<contributor>Service de Pneumologie et Allergologie ; CHU Clermont-Ferrand</contributor>
<description>International audience</description>
<source>ISSN: 1932-6203</source>
<source>PLoS ONE</source>
<publisher>Public Library of Science</publisher>
<identifier>hal-01367148</identifier>
<identifier>https://hal-univ-rennes1.archives-ouvertes.fr/hal-01367148</identifier>
<source>https://hal-univ-rennes1.archives-ouvertes.fr/hal-01367148</source>
<source>PLoS ONE, Public Library of Science, 2016, 11, pp.e0160888. 〈10.1371/journal.pone.0160888〉</source>
<identifier>DOI : 10.1371/journal.pone.0160888</identifier>
<relation>info:eu-repo/semantics/altIdentifier/doi/10.1371/journal.pone.0160888</relation>
<language>en</language>
<subject>[SDV.EE.SANT] Life Sciences [q-bio]/Ecology, environment/Health</subject>
<type>info:eu-repo/semantics/article</type>
<type>Journal articles</type>
<description lang=en>The use of recombinant antigens has been shown to improve both the sensitivity and the standardization of the serological diagnosis of Farmer's lung disease (FLD). The aim of this study was to complete the panel of recombinant antigens available for FLD serodiagnosis with antigens of Lichtheimia corymbifera, known to be involved in FLD. L. corymbifera proteins were thus separated by 2D electrophoresis and subjected to western blotting with sera from 7 patients with FLD and 9 healthy exposed controls (HEC). FLD-associated immunoreactive proteins were identified by mass spectrometry based on a protein database specifically created for this study and subsequently produced as recombinant antigens. The ability of recombinant antigens to discriminate patients with FLD from controls was assessed by ELISA performed with sera from FLD patients (n = 41) and controls (n = 43) recruited from five university hospital pneumology departments of France and Switzerland. Forty-one FLD-associated immunoreactive proteins from L. corymbifera were identified. Six of them were produced as recombinant antigens. With a sensitivity and specificity of 81.4 and 77.3% respectively, dihydrolipoyl dehydrogenase was the most effective antigen for discriminating FLD patients from HEC. ELISA performed with the putative proteasome subunit alpha type as an antigen was especially specific (88.6%) and could thus be used for FLD confirmation. The production of recombinant antigens from L. corymbifera represents an additional step towards the development of a standardized ELISA kit for FLD diagnosis. © 2016 Rognon et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.</description>
<date>2016</date>
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