untitled
<OAI-PMH schemaLocation=http://www.openarchives.org/OAI/2.0/ http://www.openarchives.org/OAI/2.0/OAI-PMH.xsd>
<responseDate>2018-01-15T18:25:29Z</responseDate>
<request identifier=oai:HAL:hal-01260141v1 verb=GetRecord metadataPrefix=oai_dc>http://api.archives-ouvertes.fr/oai/hal/</request>
<GetRecord>
<record>
<header>
<identifier>oai:HAL:hal-01260141v1</identifier>
<datestamp>2018-01-11</datestamp>
<setSpec>type:ART</setSpec>
<setSpec>subject:sdv</setSpec>
<setSpec>collection:CNRS</setSpec>
<setSpec>collection:UNIV-AG</setSpec>
<setSpec>collection:UNIV-NC</setSpec>
<setSpec>collection:EHESS</setSpec>
<setSpec>collection:IFREMER</setSpec>
<setSpec>collection:EPHE</setSpec>
<setSpec>collection:GIP-BE</setSpec>
<setSpec>collection:IRD</setSpec>
<setSpec>collection:UNIV-PERP</setSpec>
<setSpec>collection:CRIOBE</setSpec>
<setSpec>collection:AGROPOLIS</setSpec>
<setSpec>collection:PSL</setSpec>
<setSpec>collection:UNIV-POLYNESIE</setSpec>
<setSpec>collection:B3ESTE</setSpec>
<setSpec>collection:UNIV-MONTPELLIER</setSpec>
<setSpec>collection:UPF</setSpec>
<setSpec>collection:IHPE</setSpec>
<setSpec>collection:443873</setSpec>
</header>
<metadata><dc>
<publisher>HAL CCSD</publisher>
<title lang=en>Identification of genes associated with shell color in the black-lipped pearl oyster, Pinctada margaritifera.</title>
<creator>Lemer, Sarah</creator>
<creator>Saulnier, Denis</creator>
<creator>Gueguen, Yannick</creator>
<creator>Planes, Serge</creator>
<contributor>Centre de recherches insulaires et observatoire de l'environnement (CRIOBE) ; Université de Perpignan Via Domitia (UPVD) - École pratique des hautes études (EPHE) - Centre National de la Recherche Scientifique (CNRS)</contributor>
<contributor>Ecosystèmes Insulaires Océaniens (UMR 241) (EIO) ; Institut de Recherche pour le Développement (IRD) - Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER) - Institut Louis Malardé [Papeete] (ILM) ; Institut de Recherche pour le Développement (IRD) - Université de la Polynésie Française (UPF)</contributor>
<contributor>Interactions Hôtes-Pathogènes-Environnements (IHPE) ; Université de Perpignan Via Domitia (UPVD) - Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER) - Université de Montpellier (UM) - Centre National de la Recherche Scientifique (CNRS)</contributor>
<contributor>Laboratoire d'Excellence CORAIL (LabEX CORAIL) ; Institut de Recherche pour le Développement (IRD) - Université des Antilles et de la Guyane (UAG) - École des hautes études en sciences sociales (EHESS) - École pratique des hautes études (EPHE) - Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER) - Université de la Réunion (UR) - Université de la Polynésie Française (UPF) - Université de Nouvelle Calédonie - Institut d'écologie et environnement</contributor>
<description>International audience</description>
<source>ISSN: 1471-2164</source>
<source>BMC Genomics</source>
<publisher>BioMed Central</publisher>
<identifier>hal-01260141</identifier>
<identifier>https://hal.archives-ouvertes.fr/hal-01260141</identifier>
<identifier>https://hal.archives-ouvertes.fr/hal-01260141/document</identifier>
<identifier>https://hal.archives-ouvertes.fr/hal-01260141/file/Lemer-2015-BMCGenom-Identification.pdf</identifier>
<source>https://hal.archives-ouvertes.fr/hal-01260141</source>
<source>BMC Genomics, BioMed Central, 2015, 16 (1), pp.568. 〈http://link.springer.com/article/10.1186%2Fs12864-015-1776-x〉. 〈10.1186/s12864-015-1776-x〉</source>
<identifier>DOI : 10.1186/s12864-015-1776-x</identifier>
<relation>info:eu-repo/semantics/altIdentifier/doi/10.1186/s12864-015-1776-x</relation>
<source>http://link.springer.com/article/10.1186%2Fs12864-015-1776-x</source>
<language>en</language>
<subject lang=en>Differential expression</subject>
<subject lang=en> Biomineralization</subject>
<subject lang=en> Nacre</subject>
<subject lang=en> Pearl</subject>
<subject lang=en> Pigmentation</subject>
<subject lang=en> Albino</subject>
<subject>[SDV.BID.EVO] Life Sciences [q-bio]/Biodiversity/Populations and Evolution [q-bio.PE]</subject>
<type>info:eu-repo/semantics/article</type>
<type>Journal articles</type>
<description lang=en>Background: Color polymorphism in the nacre of pteriomorphian bivalves is of great interest for the pearl culture industry. The nacreous layer of the Polynesian black-lipped pearl oyster Pinctada margaritifera exhibits a large array of color variation among individuals including reflections of blue, green, yellow and pink in all possible gradients. Although the heritability of nacre color variation patterns has been demonstrated by experimental crossing, little isknown about the genes involved in these patterns. In this study, we identify a set of genes differentially expressed among extreme color phenotypes of P. margaritifera using a suppressive and subtractive hybridization (SSH) method comparing black phenotypes with full and half albino individuals.Results: Out of the 358 and 346 expressed sequence tags (ESTs) obtained by conducting two SSH libraries respectively, the expression patterns of 37 genes were tested with a real-time quantitative PCR (RT-qPCR) approach by pooling five individuals of each phenotype. The expression of 11 genes was subsequently estimated for each individual in order to detect inter-individual variation. Our results suggest that the color of the nacre is partiallyunder the influence of genes involved in the biomineralization of the calcitic layer. A few genes involved in the formation of the aragonite tablets of the nacre layer and in the biosynthesis chain of melanin also showed differential expression patterns. Finally, high variability in gene expression levels were observed within the black phenotypes.Conclusions: Our results revealed that three main genetic processes were involved in color polymorphisms: the biomineralization of the nacreous and calcitic layers and the synthesis of pigments such as melanin, suggesting that color polymorphism takes place at different levels in the shell structure. The high variability of gene expression found within black phenotypes suggests that the present work should serve as a basis for future studies exploringmore thoroughly the expression patterns of candidate genes within black phenotypes with different dominant iridescent colors.</description>
<date>2015</date>
</dc>
</metadata>
</record>
</GetRecord>
</OAI-PMH>
