untitled
<OAI-PMH schemaLocation=http://www.openarchives.org/OAI/2.0/ http://www.openarchives.org/OAI/2.0/OAI-PMH.xsd>
<responseDate>2018-01-17T12:06:04Z</responseDate>
<request identifier=oai:HAL:hal-01571305v1 verb=GetRecord metadataPrefix=oai_dc>http://api.archives-ouvertes.fr/oai/hal/</request>
<GetRecord>
<record>
<header>
<identifier>oai:HAL:hal-01571305v1</identifier>
<datestamp>2018-01-11</datestamp>
<setSpec>type:ART</setSpec>
<setSpec>subject:sdv</setSpec>
<setSpec>collection:CNRS</setSpec>
<setSpec>collection:UNIV-AG</setSpec>
<setSpec>collection:SSA</setSpec>
<setSpec>collection:AGROPOLIS</setSpec>
<setSpec>collection:UNIV-BPCLERMONT</setSpec>
<setSpec>collection:UNIV-AMU</setSpec>
<setSpec>collection:PRES_CLERMONT</setSpec>
<setSpec>collection:UNIV-CLERMONT1</setSpec>
<setSpec>collection:GRED</setSpec>
<setSpec>collection:B3ESTE</setSpec>
<setSpec>collection:UNIV-MONTPELLIER</setSpec>
</header>
<metadata><dc>
<publisher>HAL CCSD</publisher>
<title lang=en>Identification of Leishmania by MALDI-TOF mass spectrometry using a free web-based application and a dedicated mass spectral library.</title>
<creator>Lachaud, Laurence</creator>
<creator>Fernández-Arévalo, Anna</creator>
<creator>Normand, Anne Cécile</creator>
<creator>Lami, Patrick</creator>
<creator>Nabet, Cécile</creator>
<creator>Donnadieu, Jean Luc</creator>
<creator>Piarroux, Martine</creator>
<creator>Djenad, Farid</creator>
<creator>Cassagne, Carole</creator>
<creator>Ravel, Christophe</creator>
<creator>Tebar, Silvia</creator>
<creator>Llovet, Teresa</creator>
<creator>Blanchet, Denis</creator>
<creator>Demar, Magalie</creator>
<creator>Harrat, Zoubir</creator>
<creator>Aoun, Karim</creator>
<creator>Bastien, Patrick</creator>
<creator>Mu~noz, Carmen</creator>
<creator>Gallego, Montserrat</creator>
<creator>Piarroux, Renaud</creator>
<contributor>Maladies infectieuses et vecteurs : écologie, génétique, évolution et contrôle (MIVEGEC) ; Université de Montpellier (UM) - Centre National de la Recherche Scientifique (CNRS) - Institut de Recherche pour le Développement (IRD [France-Sud])</contributor>
<contributor>Sciences Economiques et Sociales de la Santé & Traitement de l'Information Médicale (SESSTIM) ; Institut de Recherche pour le Développement (IRD) - Aix Marseille Université (AMU) - ORS PACA - Institut National de la Santé et de la Recherche Médicale (INSERM)</contributor>
<contributor>Aix Marseille Université (AMU)</contributor>
<contributor>Infections Parasitaires : Transmission, Physiopathologie et Thérapeutiques (IP-TPT) ; Université de Montpellier (UM) - Service de Santé des Armées - Assistance Publique - Hôpitaux de Marseille (APHM) - Aix Marseille Université (AMU) - Institut de Recherche pour le Développement (IRD)</contributor>
<contributor>Institut Hospitalier Universitaire Méditerranée Infection (IHU AMU)</contributor>
<contributor>CNR des Leishmanioses, Montpellier</contributor>
<contributor>Centre d'Investigation Clinique Antilles-Guyane (CIC - Antilles Guyane) ; Université des Antilles et de la Guyane (UAG) - Institut National de la Santé et de la Recherche Médicale (INSERM) - CHU de Pointe-à-Pitre - Centre Hospitalier de Cayenne Andrée Rosemon - CHU de Fort de France</contributor>
<contributor>Laboratoire Hospitalo-Universitaire de Parasitologie-Mycologie [Cayenne, Guyane Française] ; Centre Hospitalier de Cayenne Andrée Rosemon - Coordination Régionale de la lutte contre le Virus de L'Immunodéficience Humaine (COREVIH)</contributor>
<contributor>Institut Pasteur d'Algérie</contributor>
<contributor>Université des Sciences et de la Technologie Houari Boumediene [Alger] (USTHB)</contributor>
<contributor>Laboratoire de recherche parasitoses médicales, Biotechnologie & Biomolécules, LR 11 IPT 06, Institut Pasteur de Tunis, Tunis, Tunisia.</contributor>
<contributor>Laboratoire de Parasitologie-Mycologie, Montpellier (MIVEGEC) ; Université Montpellier 1 (UM1) - Université Montpellier 2 - Sciences et Techniques (UM2) - Centre Hospitalier Régional Universitaire [Montpellier] (CHRU Montpellier) - Institut de recherche pour le développement [IRD] : UR224 - Université de Montpellier (UM) - Centre National de la Recherche Scientifique (CNRS)</contributor>
<contributor>Centre National de référence de la Toxoplasmose (CNRT)</contributor>
<contributor>Génétique, reproduction et développement (GReD) ; Centre National de la Recherche Scientifique (CNRS) - Institut National de la Santé et de la Recherche Médicale (INSERM) - IFR79 - Université d'Auvergne - Clermont-Ferrand I (UdA) - Université Blaise Pascal - Clermont-Ferrand 2 (UBP)</contributor>
<contributor>Assistance Publique - Hôpitaux de Marseille (APHM)</contributor>
<description>International audience</description>
<source>ISSN: 0095-1137</source>
<source>Journal of Clinical Microbiology</source>
<publisher>American Society for Microbiology</publisher>
<identifier>hal-01571305</identifier>
<identifier>https://hal.archives-ouvertes.fr/hal-01571305</identifier>
<source>https://hal.archives-ouvertes.fr/hal-01571305</source>
<source>Journal of Clinical Microbiology, American Society for Microbiology, 2017, pp.JCM.00845--17. 〈10.1128/JCM.00845-17〉</source>
<identifier>DOI : 10.1128/JCM.00845-17</identifier>
<relation>info:eu-repo/semantics/altIdentifier/doi/10.1128/JCM.00845-17</relation>
<identifier>PUBMED : 28724559</identifier>
<relation>info:eu-repo/semantics/altIdentifier/pmid/28724559</relation>
<language>en</language>
<subject>[SDV.MHEP] Life Sciences [q-bio]/Human health and pathology</subject>
<type>info:eu-repo/semantics/article</type>
<type>Journal articles</type>
<description lang=en>Human leishmaniasis are widely spread diseases with different clinical forms caused by about 20 species within the Leishmania genus. Leishmania species identification is relevant for therapeutic management and prognosis, especially for cutaneous and muco-cutaneous forms. Several methods are available to identify Leishmania species from culture, but they have not been standardized for the majority of the currently described species, with the exception of MultiLocus Enzyme Electrophoresis. Moreover, these techniques are expensive, time-consuming and not available in all laboratories. Within the last decade, mass spectrometry (MS) has been adapted for microorganism identification, including Leishmania However, no commercial reference mass spectral database is available. In this study, a reference mass spectral library (MSL) for Leishmania isolates, accessible through a free web-based application (mass spectral identification, MSI), was constructed and tested. It includes mass spectral data for 33 different Leishmania species, including species that infect humans, animals and phlebotomine vectors. Four laboratories on two continents evaluated MSI performance using 268 samples of which 231 were Leishmania strains. All Leishmania strains, but one, were correctly identified at least at the complex level. A risk of species misidentification within the L. donovani, L. guyanensis and L. braziliensis complexes was observed, as already reported with other techniques. The tested application was reliable, with identification results comparable to those obtained with reference methods, but with a more favorable cost-efficiency ratio. This free online identification system relies on a scalable database and can be implemented directly in the user's computers.</description>
<date>2017-07</date>
</dc>
</metadata>
</record>
</GetRecord>
</OAI-PMH>
