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<datestamp>2018-01-11</datestamp>
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<title lang=en>Assessment of a simple compound-saving method to study insecticidal activity of natural extracts and pure compounds against mosquito larvae</title>
<creator>Falkowski, Michaël</creator>
<creator>Jahn-Oyac, Arnaud</creator>
<creator>Ferrero, Emma</creator>
<creator>Issaly, Jean</creator>
<creator>Eparvier, Veronique</creator>
<creator>Girod, Romain</creator>
<creator>Rodrigues, Alice M. S.</creator>
<creator>Stien, Didier</creator>
<creator>Houel, Emeline</creator>
<creator>Dusfour, Isabelle</creator>
<contributor>Ecologie des forêts de Guyane (ECOFOG) ; Centre de Coopération Internationale en Recherche Agronomique pour le Développement (CIRAD) - Institut National de la Recherche Agronomique (INRA) - Université des Antilles et de la Guyane (UAG) - AgroParisTech - Université de Guyane (UG) - Centre National de la Recherche Scientifique (CNRS)</contributor>
<contributor>Université de Guyane (UG)</contributor>
<contributor>Université des Antilles (UA)</contributor>
<contributor>Centre National de la Recherche Scientifique (CNRS)</contributor>
<contributor>Centre de Coopération Internationale en Recherche Agronomique pour le Développement</contributor>
<contributor>AgroParisTech</contributor>
<contributor>Unité d'Entomologie Médicale [Antananarivo, Madagascar] (IPM) ; Institut Pasteur de Madagascar - Réseau International des Instituts Pasteur (RIIP)</contributor>
<contributor>UPR2301, Institut de Chimie des Substances Naturelles (ICSN) ; Centre National de la Recherche Scientifique (CNRS)</contributor>
<contributor>Laboratoire de Biodiversité et Biotechnologies Microbiennes (LBBM) ; Université Pierre et Marie Curie - Paris 6 (UPMC) - PIERRE FABRE - Electricité de France Recherche et Développement (EDF R&D) - Centre National de la Recherche Scientifique (CNRS)</contributor>
<contributor>Polytech'Paris-UPMC ; Université Pierre et Marie Curie - Paris 6 (UPMC)</contributor>
<contributor>Sorbonne Universités</contributor>
<contributor>Observatoire Océanologique</contributor>
<contributor>Europe (ERDF OP); French Guiana Regional Council; Air Liquide Foundation; "Investissement d'Avenir" grant [ANR-10-LABX-25-01]; European commission [REGPOT-CT-2011-285837-STRonGer]</contributor>
<source>ISSN: 8756-971X</source>
<source>Journal of the American Mosquito Control Association</source>
<publisher>American Mosquito Control Association</publisher>
<identifier>hal-01602207</identifier>
<identifier>https://hal.archives-ouvertes.fr/hal-01602207</identifier>
<source>https://hal.archives-ouvertes.fr/hal-01602207</source>
<source>Journal of the American Mosquito Control Association, American Mosquito Control Association, 2016, 32 (4), pp.337-340. 〈10.2987/16-6613.1〉</source>
<identifier>DOI : 10.2987/16-6613.1</identifier>
<relation>info:eu-repo/semantics/altIdentifier/doi/10.2987/16-6613.1</relation>
<identifier>PRODINRA : 394297</identifier>
<identifier>PUBMED : 28206865</identifier>
<relation>info:eu-repo/semantics/altIdentifier/pmid/28206865</relation>
<language>en</language>
<subject lang=en>Mosquitoes</subject>
<subject lang=en>natural insecticides</subject>
<subject lang=en>screening method</subject>
<subject>[SDV] Life Sciences [q-bio]</subject>
<type>info:eu-repo/semantics/article</type>
<type>Journal articles</type>
<description lang=en>Research on natural insecticides has intensified with the spread of resistance to chemicals among insects, particularly disease vectors. To evaluate compounds, the World Health Organization (WHO) has published standardized procedures. However, those may be excessively compound-consuming when it comes to assessing the activity of natural extracts and pure compounds isolated in limited amount. As part of our work on the discovery of new mosquito larvicides from Amazonian plants, we developed a compound-saving assay in 5-ml glass tubes instead of WHO larval 100-m1 cups. Comparing activity of synthetic and natural chemicals validated the glass tube assay. Raw data, lethal doses that kill 50% (LD50) and 90% (LD90) at 24 and 48 h, were highly correlated (0.68 < R-2 < 0.96, P < 0.001, Pearson test) between cups and tubes. It was also established that 10 tubes (N = 50 larvae) provided the same level of sensitivity as 20 tubes (N= 100). This method proved suitable for rapid screening of natural extracts and molecules, identifying active compounds using 10 times less material than in the WHO protocol.</description>
<date>2016</date>
</dc>
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