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<title lang=en>Ameloblasts Express Type I Collagen during Amelogenesis</title>
<creator>Assaraf-Weill, N.</creator>
<creator>Gasse, B.</creator>
<creator>Silvent, J.</creator>
<creator>Bardet, C.</creator>
<creator>Sire, J-Y.</creator>
<creator>Davit-Beal, T.</creator>
<contributor>Evolution et développement du squelette (EDS) ; Systématique, adaptation, évolution (SAE) ; Centre National de la Recherche Scientifique (CNRS) - Université Pierre et Marie Curie - Paris 6 (UPMC) - Centre National de la Recherche Scientifique (CNRS) - Université Pierre et Marie Curie - Paris 6 (UPMC) - Evolution Paris Seine ; Université des Antilles et de la Guyane (UAG) - Université Pierre et Marie Curie - Paris 6 (UPMC) - Université Nice Sophia Antipolis (UNS) ; Université Côte d'Azur (UCA) - Université Côte d'Azur (UCA) - Centre National de la Recherche Scientifique (CNRS) - Université des Antilles et de la Guyane (UAG) - Université Nice Sophia Antipolis (UNS) ; Université Côte d'Azur (UCA) - Université Côte d'Azur (UCA)</contributor>
<contributor>CNRS</contributor>
<contributor> Universite Pierre Marie Curie</contributor>
<description>International audience</description>
<source>ISSN: 0022-0345</source>
<source>Journal of Dental Research</source>
<publisher>SAGE Publications (UK and US)</publisher>
<identifier>hal-01545475</identifier>
<identifier>https://hal.archives-ouvertes.fr/hal-01545475</identifier>
<source>https://hal.archives-ouvertes.fr/hal-01545475</source>
<source>Journal of Dental Research, SAGE Publications (UK and US), 2014, 93 (5), pp.502-507. 〈10.1177/0022034514526236〉</source>
<identifier>DOI : 10.1177/0022034514526236</identifier>
<relation>info:eu-repo/semantics/altIdentifier/doi/10.1177/0022034514526236</relation>
<language>en</language>
<subject lang=en>enameloid</subject>
<subject lang=en> Pleurodeles waltl</subject>
<subject lang=en> amphibian</subject>
<subject lang=en> in situ hybridization</subject>
<subject lang=en> amelogenin</subject>
<subject lang=en> odontogenesis</subject>
<subject>[SDV.BID.EVO] Life Sciences [q-bio]/Biodiversity/Populations and Evolution [q-bio.PE]</subject>
<type>info:eu-repo/semantics/article</type>
<type>Journal articles</type>
<description lang=en>Enamel and enameloid, the highly mineralized tooth-covering tissues in living vertebrates, are different in their matrix composition. Enamel, a unique product of ameloblasts, principally contains enamel matrix proteins (EMPs), while enameloid possesses collagen fibrils and probably receives contributions from both odontoblasts and ameloblasts. Here we focused on type I collagen (COL1A1) and amelogenin (AMEL) gene expression during enameloid and enamel formation throughout ontogeny in the caudate amphibian, Pleurodeles waltl. In this model, pre-metamorphic teeth possess enameloid and enamel, while post-metamorphic teeth possess enamel only. In first-generation teeth, qPCR and in situ hybridization (ISH) on sections revealed that ameloblasts weakly expressed AMEL during late-stage enameloid formation, while expression strongly increased during enamel deposition. Using ISH, we identified COL1A1 transcripts in ameloblasts and odontoblasts during enameloid formation. COL1A1 expression in ameloblasts gradually decreased and was no longer detected after metamorphosis. The transition from enameloid-rich to enamel-rich teeth could be related to a switch in ameloblast activity from COL1A1 to AMEL synthesis. P. waltl therefore appears to be an appropriate animal model for the study of the processes involved during enameloid-to-enamel transition, especially because similar events probably occurred in various lineages during vertebrate evolution.</description>
<date>2014-05</date>
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