Audio-Vidéo : cfcs-1207-14
Development of phytotoxicity bioassay for soil amendment products from organic waste recycling
Auteur(s) : Lopez, Francis
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: University of West Indies, Barbados Université des Antilles. Service commun de la documentation
Extrait de : 52e congrès annuel de la Société caribéenne des plantes alimentaires / 52nd annual meeting of the Caribbean food crops society (CFCS), du 10 au 16 juillet 2016. INRA, CFCS
Description : A germination bioassay was developed to screen for potential phytotoxicity of soil amendment products from organic waste recycling. In phase 1 of the study, nine soil amendment products were applied as mulches on 3- to 5-week-old tomato seedlings (Solanum lycopersicum L.HA3019?) planted in 4L pots. Plant growth was reduced by the less mature mulch materials. Using lettuce (Lactuca sativa L.New Red Fire?) as the test species, a laboratory germination test was developed in phase 2 with aqueous extracts from a non-phytotoxic (safe) and a phytotoxic product identified in phase 1. Extracts were obtained by soaking 10g of air-dried product in 90 mL distilled water for 24h at 25°C. Diluted extracts (0, 25, 50, 75 and 100%) were applied to lettuce seeds sown on filter paper in petri-dishes and germination was monitored for 5 days, confirming the results from phase 1. In phase 3, the bioassay developed in phase 2 was used to test three previously untested local products and one imported product. Two of the local products were comparable to the imported product. Results obtained in phase 3 were confirmed using the mulch test as described in phase 1. Hence, the germination bioassay can be a useful tool for quality control in the production of(safe) soil amendment products from organic waste recycling.
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